Adenovirus Type 12 Transformation Involves Loss of Beta-adrenergic Receptors and Isoproterenol Responsiveness

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1982 Jul 1

PMID 6152838

Authors

N Ledinko

J Schaeufele

C Chen

Affiliations

Soon will be listed here.

Abstract

The responsiveness of a growth-regulated rat 3Y1 cell line and five clones of 3Y1 cells transformed by the highly oncogenic human adenovirus type 12 to the catecholamine hormone (-)-isoproterenol was studied. The untransformed cells contained beta-adrenergic receptors characterized by specific binding of the beta-adrenergic receptor antagonist (-)-[3H]dihydroalprenolol, a 9- to 12-fold increase in cyclic AMP production in intact cells after incubation with 10 microM (-)-isoproterenol, and significantly increased adenylate cyclase (ATP pyrophosphatelyase [cyclizing], EC 4.6.1.1) activity in the presence of the hormone. In contrast, (-)-isoproterenol (10 to 100 microM) had no apparent effect on cyclic AMP production or the basal adenylate cyclase activity in the transformed cell lines. Binding studies revealed that untransformed cells contained approximately 19,400 beta-adrenergic receptor sites per cell. Three transformed cell clones tested showed a three- to fourfold loss of beta-adrenergic receptors.

References

Brooker G, Harper J, Terasaki W, Moylan R . Radioimmunoassay of cyclic AMP and cyclic GMP. Adv Cyclic Nucleotide Res. 1979; 10:1-33. View

KLAINER L, CHI Y, FREIDBERG S, RALL T, SUTHERLAND E . Adenyl cyclase. IV. The effects of neurohormones on the formation of adenosine 3',5'-phosphate by preparations from brain and other tissues. J Biol Chem. 1962; 237:1239-43. View

Nielson S, PUCK T . Deposition of fibronectin in the course of reverse transformation of Chinese hamster ovary cells by cyclic AMP. Proc Natl Acad Sci U S A. 1980; 77(2):985-9. PMC: 348408. DOI: 10.1073/pnas.77.2.985. View

Zadunaisky J, Schaeffer B, Cherksey B . Chloride active transport, membrane lipids and receptors in the corneal epithelium. Ann N Y Acad Sci. 1980; 341:233-45. DOI: 10.1111/j.1749-6632.1980.tb47175.x. View

Fisher P, Lee L, Weinstein I . Changes in epidermal growth factor receptors associated with adenovirus transformation, chemical carcinogen transformation and exposure to a phorbol ester tumor promoter. Biochem Biophys Res Commun. 1980; 93(4):1160-6. DOI: 10.1016/0006-291x(80)90611-7. View

Blomberg J, Reynolds Jr F, Van de Ven W, Stephenson J . Abelson murine leukaemia virus transformation involves loss of epidermal growth factor-binding sites. Nature. 1980; 286(5772):504-7. DOI: 10.1038/286504a0. View

Cherksey B, Zadunaisky J, Murphy R . Cytoskeletal constraint of the beta-adrenergic receptor in frog erythrocyte membranes. Proc Natl Acad Sci U S A. 1980; 77(11):6401-5. PMC: 350292. DOI: 10.1073/pnas.77.11.6401. View

Levitzki A . The beta-adrenergic receptor and its mode of coupling to adenylate cyclase. CRC Crit Rev Biochem. 1981; 10(2):81-112. DOI: 10.3109/10409238109114550. View

Garte S, BELMAN S . Decreased beta-adrenergic responsiveness in mouse epidermal papillomas during tumor promotion with phorbol myristate acetate. Cancer Lett. 1980; 9(3):245-9. DOI: 10.1016/0304-3835(80)90094-4. View

10.

Ledinko N, Bhe F . Adenovirus type 12 gene 401 function and temperature sensitivity of cytochalasin B effects on transformed cells. Intervirology. 1980; 14(5-6):253-60. DOI: 10.1159/000149194. View

11.

Hsie A, PUCK T . Morphological transformation of Chinese hamster cells by dibutyryl adenosine cyclic 3':5'-monophosphate and testosterone. Proc Natl Acad Sci U S A. 1971; 68(2):358-61. PMC: 388937. DOI: 10.1073/pnas.68.2.358. View

12.

Brown B, Albano J, Ekins R, Sgherzi A . A simple and sensitive saturation assay method for the measurement of adenosine 3':5'-cyclic monophosphate. Biochem J. 1971; 121(3):561-2. PMC: 1176606. DOI: 10.1042/bj1210561. View

13.

Sheppard J . Restoration of contact-inhibited growth to transformed cells by dibutyryl adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1971; 68(6):1316-20. PMC: 389180. DOI: 10.1073/pnas.68.6.1316. View

14.

SHEPPARD H, Wiggan G . Different sensitivities of the phosphodiesterases (adenosine-3',5'-cyclic phosphate 3'-phosphohydrolase) of dog cerebral cortex and erythrocytes to inhibition by synthetic agents and cold. Biochem Pharmacol. 1971; 20(8):2128-30. DOI: 10.1016/0006-2952(71)90426-6. View

15.

Frank W . Cyclic 3':5' AMP and cell proliferation in cultures of embryonic rat cells. Exp Cell Res. 1972; 71(1):238-41. DOI: 10.1016/0014-4827(72)90287-x. View

16.

Johnson G, Pastan I . Role of 3',5'-adenosine monophosphate in regulation of morphology and growth of transformed and normal fibroblasts. J Natl Cancer Inst. 1972; 48(5):1377-87. View

17.

Willingham M, Johnson G, Pastan I . Control of DNA synthesis and mitosis in 3T3 cells by cyclic AMP. Biochem Biophys Res Commun. 1972; 48(4):743-8. DOI: 10.1016/0006-291x(72)90669-9. View

18.

McNutt N, Culp L, Black P . Contact-inhibited revertant cell lines isolated from SV 40-transformed cells. IV. Microfilament distribution and cell shape in untransformed, transformed, and revertant Balb-c 3T3 cells. J Cell Biol. 1973; 56(2):412-28. PMC: 2108904. DOI: 10.1083/jcb.56.2.412. View

19.

Froehlich J, Rachmeler M . Effect of adenosine 3'-5'-cyclic monophosphate on cell proliferation. J Cell Biol. 1972; 55(1):19-31. PMC: 2108750. DOI: 10.1083/jcb.55.1.19. View

20.

Froehlich J, Rachmeler M . Inhibition of cell growth in the G1 phase by adenosine 3', 5'-cyclic monophosphate. J Cell Biol. 1974; 60(1):249-57. PMC: 2109140. DOI: 10.1083/jcb.60.1.249. View