Control of Peptide Release from Cells of the Intermediate Lobe of the Rat Pituitary

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1984 Jan 1

PMID 6147194

Citations 1

Authors

G Stoll

R Martin

K H Voigt

Affiliations

Soon will be listed here.

Abstract

When rats were injected with 6-hydroxydopamine the catecholaminergic nerve terminals in their intermediate lobes exhibited distinct signs of degeneration. Morphometric examination of the Golgi apparatus in cells of the intermediate lobe of these rats showed significant enlargement of Golgi cisternae. The release of adrenocorticotropin, beta-endorphin/lipotropin and alpha-melanotropin from intermediate-lobe cells in vitro was measured by radioimmunoassay. The high basal peptide release was inhibited by dopamine and stimulated by methyl-isobutyl-xanthine. In contrast, gamma-aminobutyric acid, serotonin, histamine and noradrenaline, or corticotropin-releasing hormone, rat hypothalamic extract and vasopressin had no or only very weak effects. These observations indicate that the synthetic apparatus of intermediate-lobe cells is constantly depressed by dopaminergic nerves. We were not able to stimulate peptide release from intermediate-lobe cells by use of the above-mentioned endogenous agents.

Citing Articles

The hypothalamo-hypophyseal rat explant in vitro: endocrinological studies of the pars intermedia dopaminergic neural input.

Davis M J Physiol. 1986; 370:381-93.

PMID: 3958980 PMC: 1192686. DOI: 10.1113/jphysiol.1986.sp015940.

References

Tilders F, Mulder A, Smelik P . On the presence of a MSH-release inhibiting system in the rat neurointermediate lobe. Neuroendocrinology. 1975; 18(1):125-30. DOI: 10.1159/000122390. View

Bower A, Hadley M, Hruby V . Biogenic amines and control of melanophore stimulating hormone release. Science. 1974; 184(4132):70-2. DOI: 10.1126/science.184.4132.70. View

Labrie F, Dumont D, Cote J, Coy D, Sveiraf J . Corticotropin-releasing factor stimulates secretion of melanocyte-stimulating hormone from the rat pituitary. Science. 1982; 217(4554):62-3. DOI: 10.1126/science.6283632. View

Jackson S, Lowry P . Distribution of adrenocorticotrophic and lipotrophic peptides in the rat. J Endocrinol. 1980; 86(2):205-19. DOI: 10.1677/joe.0.0860205. View

Rossier J, FRENCH E, Gros C, Minick S, Guillemin R, Bloom F . Adrenalectomy, dexamethasone or stress alters opioid peptides levels in rat anterior pituitary but not intermediate lobe or brain. Life Sci. 1979; 25(24-25):2105-12. DOI: 10.1016/0024-3205(79)90204-2. View

Tomiko S, Taraskevich P, DOUGLAS W . GABA acts directly on cells of pituitary pars intermedia to alter hormone output. Nature. 1983; 301(5902):706-7. DOI: 10.1038/301706a0. View

Volcanes B, Weatherhead B . Stereological analysis of the effects of 6-hydroxydopamine on the ultrastructure of the melanocyte-stimulating hormone cell of the pars intermedia of the pituitary of Xenopus laevis. Gen Comp Endocrinol. 1976; 28(2):205-12. DOI: 10.1016/0016-6480(76)90171-4. View

Chen C, Dionne F, Roberts J . Regulation of the pro-opiomelanocortin mRNA levels in rat pituitary by dopaminergic compounds. Proc Natl Acad Sci U S A. 1983; 80(8):2211-5. PMC: 393788. DOI: 10.1073/pnas.80.8.2211. View

Oertel W, Mugnaini E, Tappaz M, Weise V, Dahl A, Schmechel D . Central GABAergic innervation of neurointermediate pituitary lobe: biochemical and immunocytochemical study in the rat. Proc Natl Acad Sci U S A. 1982; 79(2):675-9. PMC: 345809. DOI: 10.1073/pnas.79.2.675. View

10.

Baumgarten H, Bjorklund A, HOLSTEIN A, Nobin A . Organization and ultrastructural identification of the catecholamine nerve terminals in the neural lobe and pars intermedia of the rat pituitary. Z Zellforsch Mikrosk Anat. 1972; 126(4):483-517. DOI: 10.1007/BF00306908. View

11.

Sachs C, Jonsson G . Mechanisms of action of 6-hydroxydopamine. Biochem Pharmacol. 1975; 24(1):1-8. DOI: 10.1016/0006-2952(75)90304-4. View

12.

Breese G, Traylor T . Effect of 6-hydroxydopamine on brain norepinephrine and dopamine evidence for selective degeneration of catecholamine neurons. J Pharmacol Exp Ther. 1970; 174(3):413-20. PMC: 3055658. View

13.

Portanova R, Smith D, Sayers G . A trypsin technic for the preparation of isolated rat anterior pituitary cells. Proc Soc Exp Biol Med. 1970; 133(2):573-6. DOI: 10.3181/00379727-133-34520. View

14.

Smyth D, ZAKARIAN S . Selective processing of beta-endorphin in regions of porcine pituitary. Nature. 1980; 288(5791):613-5. DOI: 10.1038/288613a0. View

15.

Tilders F, van der Woude H, Swaab D, Mulder A . Identification of MSH release-inhibiting elements in the neurointermediate lobe of the rat. Brain Res. 1979; 171(3):425-35. DOI: 10.1016/0006-8993(79)91047-3. View

16.

Tranzer J, Thoenen H . An electron microscopic study of selective, acute degeneration of sympathetic nerve terminals after administration of 6-hydroxydopamine. Experientia. 1968; 24(2):155-6. DOI: 10.1007/BF02146956. View

17.

Herbert E, Birnberg N, Civelli O, Lissitzky J, Uhler M, Durrin L . Regulation of genetic expression of pro-opiomelanocortin in pituitary and extrapituitary tissues of mouse and rat. Adv Biochem Psychopharmacol. 1982; 33:9-18. View

18.

Martin R, Weber E, Voigt K . Localization of corticotropin- and endorphin-related peptides in the intermediate lobe of the rat pituitary. Cell Tissue Res. 1979; 196(2):307-19. DOI: 10.1007/BF00240104. View

19.

Meunier H, Labrie F . The dopamine receptor in the intermediate lobe of the rat pituitary gland is negatively coupled to adenylate cyclase. Life Sci. 1982; 30(11):963-8. DOI: 10.1016/0024-3205(82)90625-7. View

20.

Gibbs D, Stewart R, Liu J, Vale W, Rivier J, Yen S . Effects of synthetic corticotropin-releasing factor and dopamine on the release of immunoreactive beta-endorphin/beta-lipotropin and alpha-melanocyte-stimulating hormone from human fetal pituitaries in vitro. J Clin Endocrinol Metab. 1982; 55(6):1149-52. DOI: 10.1210/jcem-55-6-1149. View