Effect of Lodoxamide on the Secretory Response Induced by Escherichia Coli and Vibrio Cholerae Enterotoxins in Infant Mice

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1984 May 1

PMID 6143729

Citations 2

Authors

F C Knoop

D D Thomas

Affiliations

Soon will be listed here.

Abstract

The effect of lodoxamide tromethamine, a calcium antagonist, on intestinal fluid accumulation induced by Escherichia coli heat-stable (ST) and Vibrio cholerae (CT) enterotoxins in infant mice was investigated. The simultaneous administration of lodoxamide with ST or CT enterotoxin resulted in a significant (P less than 0.01) inhibition of the intestinal fluid response. A minimum concentration of 10(-7) or 10(-8)M lodoxamide caused an inhibition (P less than 0.01) of the ST- or CT-mediated fluid response, respectively. Treatment of infant mice with buffer or drug alone did not result in fluid accumulation. A significant inhibition of ST and CT enterotoxic activities was also observed when lodoxamide was administered 30 min before (P less than 0.02) or 30 min after (P less than 0.01) toxin challenge. These data suggest that calcium may be important in the ST- or CT-mediated induction of fluid accumulation. Further studies on the potential use of lodoxamide tromethamine in both the prophylaxis and treatment of diarrheal disease appear warranted.

Citing Articles

Enteric bacterial toxins: mechanisms of action and linkage to intestinal secretion.

Sears C, Kaper J Microbiol Rev. 1996; 60(1):167-215.

PMID: 8852900 PMC: 239424. DOI: 10.1128/mr.60.1.167-215.1996.

Evaluation of BW942C, a novel antidiarrheal agent, against enterotoxins of Escherichia coli and Vibrio cholerae.

Morgan D, Sellin J, Gutierrez L, DuPont H, Wood L Infect Immun. 1985; 48(3):754-8.

PMID: 3838969 PMC: 261252. DOI: 10.1128/iai.48.3.754-758.1985.

References

. Field M: Intestinal secretion: effect of cyclic AMP and its role in cholera. N Engl J Med. 1971; 284(20):1137-44. DOI: 10.1056/NEJM197105202842008. View

SCHAFER D, Lust W, Sircar B, Goldberg N . Elevated concentration of adenosine 3':5'-cyclic monophosphate in intestinal mucosa after treatment with cholera toxin. Proc Natl Acad Sci U S A. 1970; 67(2):851-6. PMC: 283283. DOI: 10.1073/pnas.67.2.851. View

JACOBY H, Marshall C . Antagonism of cholera enterotoxin by anti-inflammatory agents in the rat. Nature. 1972; 235(5334):163-5. DOI: 10.1038/235163a0. View

Dean A, Ching Y, Williams R, Harden L . Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972; 125(4):407-11. DOI: 10.1093/infdis/125.4.407. View

Cuatrecasas P . Interaction of Vibrio cholerae enterotoxin with cell membranes. Biochemistry. 1973; 12(18):3547-58. DOI: 10.1021/bi00742a031. View

Baselski V, Briggs R, Parker C . Intestinal fluid accumulation induced by oral challenge with Vibrio cholerae or cholera toxin in infant mice. Infect Immun. 1977; 15(3):704-12. PMC: 421427. DOI: 10.1128/iai.15.3.704-712.1977. View

Evans Jr D, Evans D, DuPont H, ORSKOV F, ORSKOV I . Patterns of loss of enterotoxigenicity by Escherichia coli isolated from adults with diarrhea: suggestive evidence for an interrelationship with serotype. Infect Immun. 1977; 17(1):105-11. PMC: 421088. DOI: 10.1128/iai.17.1.105-111.1977. View

Bolton J, Field M . Ca ionophore-stimulated ion secretion in rabbit ileal mucosa: relation to actions of cyclic 3',5'-AMP and carbamylcholine. J Membr Biol. 1977; 35(2):159-73. DOI: 10.1007/BF01869947. View

Gill D . Mechanism of action of cholera toxin. Adv Cyclic Nucleotide Res. 1977; 8:85-118. View

10.

Cassel D, Pfeuffer T . Mechanism of cholera toxin action: covalent modification of the guanyl nucleotide-binding protein of the adenylate cyclase system. Proc Natl Acad Sci U S A. 1978; 75(6):2669-73. PMC: 392624. DOI: 10.1073/pnas.75.6.2669. View

11.

Gill D, Meren R . ADP-ribosylation of membrane proteins catalyzed by cholera toxin: basis of the activation of adenylate cyclase. Proc Natl Acad Sci U S A. 1978; 75(7):3050-4. PMC: 392711. DOI: 10.1073/pnas.75.7.3050. View

12.

Vaughan M, Moss J . Mechanism of action of choleragen. J Supramol Struct. 1978; 8(4):473-88. DOI: 10.1002/jss.400080410. View

13.

Madsen G, Knoop F . Inhibition of the secretory activity of Escherichia coli heat-stable enterotoxin by indomethacin. Infect Immun. 1978; 22(1):143-7. PMC: 422128. DOI: 10.1128/iai.22.1.143-147.1978. View

14.

Newsome P, Burgess M, Mullan N . Effect of Escherichia coli heat-stable enterotoxin on cyclic GMP levels in mouse intestine. Infect Immun. 1978; 22(1):290-1. PMC: 422149. DOI: 10.1128/iai.22.1.290-291.1978. View

15.

Field M . Mechanisms of action of cholera and Escherichia coli enterotoxins. Am J Clin Nutr. 1979; 32(1):189-96. DOI: 10.1093/ajcn/32.1.189. View

16.

Donowitz M, Charney A . Propranolol prevention of cholera enterotoxin-induced intestinal secretion in the rat. Gastroenterology. 1979; 76(3):482-91. View

17.

Frizzell R, Smith P, Vosburgh E, Field M . Coupled sodium-chloride influx across brush border of flounder intestine. J Membr Biol. 1979; 46(1):27-39. DOI: 10.1007/BF01959973. View

18.

Knoop F, Abbey D . A possible role for calcium in cyclic nucleotide mediated fluid secretion. Med Hypotheses. 1981; 7(6):719-27. DOI: 10.1016/0306-9877(81)90083-9. View

19.

Brostrom M, Brostrom C, Huang S, Wolff D . Cholera toxin-stimulated cyclic AMP accumulation in glial tumor cells: modulation by Ca2+. Mol Pharmacol. 1981; 20(1):59-67. View

20.

Knoop F, Abbey D . Effect of chemical and pharmacological agents on the secretory activity induced by Escherichia coli heat-stable enterotoxin. Can J Microbiol. 1981; 27(8):754-8. DOI: 10.1139/m81-117. View