Different Exon-intron Organization at the 5' Part of a Mouse Class I Gene is Used to Generate a Novel H-2Kd-related MRNA

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1983 Dec 1

PMID 6143316

Citations 15

Authors

J L Lalanne

M Cochet

A M Kummer

G Gachelin

P Kourilsky

Affiliations

Soon will be listed here.

Abstract

A cDNA library constructed from liver mRNA of DBA/2 (H-2d) mice has been screened with H-2-specific probes. The nucleotide sequence of one clone (pH-2d-24) indicates that it derives from an H-2 gene with an unexpected exon-intron organization. Nucleotide sequence comparisons suggest that two distinct mRNAs are produced from a single H-2Kd gene by a mechanism involving the use of alternative splicing sites in its 5' region. pH-2d-24 carries an open reading frame encoding a thus-far-undescribed polypeptide product identical to an H-2Kd-molecule, except for the NH2-terminal half of the first domain.

Citing Articles

Serological and immunochemical analysis of H-2 class I molecules encoded by the Db region.

Mierau R, Cramer M Immunogenetics. 1984; 20(3):341-5.

PMID: 6469291 DOI: 10.1007/BF00364215.

Alternative splicing in the 5' moiety of the H-2Kd gene transcript.

Transy C, Lalanne J, Kourilsky P EMBO J. 1984; 3(10):2383-6.

PMID: 6094182 PMC: 557697. DOI: 10.1002/j.1460-2075.1984.tb02143.x.

DNA sequence of the mouse H-2Dd transplantation antigen gene.

Sher B, Nairn R, Coligan J, HOOD L Proc Natl Acad Sci U S A. 1985; 82(4):1175-9.

PMID: 3856254 PMC: 397217. DOI: 10.1073/pnas.82.4.1175.

Two subtypes of BfF by isoelectrofocusing: differential linkage to other HLA markers.

Abbal M, Thomsen M, Cambon-Thomsen A, Archambeau J, Calot M, Fathallah D Hum Genet. 1985; 69(2):181-3.

PMID: 3855837 DOI: 10.1007/BF00293294.

An H-2K gene of the tw32 mutant at the T/t complex is a close parent of an H-2Kq gene.

Morita T, Delarbre C, Kress M, Kourilsky P, Gachelin G Immunogenetics. 1985; 21(4):367-83.

PMID: 3838967 DOI: 10.1007/BF00430802.

References

Kvist S, Roberts L, Dobberstein B . Mouse histocompatibility genes: structure and organisation of a Kd gene. EMBO J. 1983; 2(2):245-54. PMC: 555121. DOI: 10.1002/j.1460-2075.1983.tb01413.x. View

Weiss E, Golden L, Zakut R, Mellor A, Fahrner K, Kvist S . The DNA sequence of the H-2kb gene: evidence for gene conversion as a mechanism for the generation of polymorphism in histocompatibilty antigens. EMBO J. 1983; 2(3):453-62. PMC: 555154. DOI: 10.1002/j.1460-2075.1983.tb01444.x. View

Sirover M, Loeb L . On the fidelity of DNA replication. Effect of metal activators during synthesis with avian myeloblastosis virus DNA polymerase. J Biol Chem. 1977; 252(11):3605-10. View

Breathnach R, Benoist C, OHare K, Gannon F, Chambon P . Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries. Proc Natl Acad Sci U S A. 1978; 75(10):4853-7. PMC: 336219. DOI: 10.1073/pnas.75.10.4853. View

Rogers J, Early P, Carter C, Calame K, Bond M, Hood L . Two mRNAs with different 3' ends encode membrane-bound and secreted forms of immunoglobulin mu chain. Cell. 1980; 20(2):303-12. DOI: 10.1016/0092-8674(80)90616-9. View

Young R, Hagenbuchle O, Schibler U . A single mouse alpha-amylase gene specifies two different tissue-specific mRNAs. Cell. 1981; 23(2):451-8. DOI: 10.1016/0092-8674(81)90140-9. View

Demant P, Ivanyi D . Further molecular complexities of H-2 K- and D-region antigens. Nature. 1981; 290(5802):146-9. DOI: 10.1038/290146a0. View

Go M . Correlation of DNA exonic regions with protein structural units in haemoglobin. Nature. 1981; 291(5810):90-2. DOI: 10.1038/291090a0. View

Klein J, Juretic A, Baxevanis C, Nagy Z . The traditional and a new version of the mouse H-2 complex. Nature. 1981; 291(5815):455-60. DOI: 10.1038/291455a0. View

10.

Steinmetz M, Frelinger J, Fisher D, Hunkapiller T, Pereira D, Weissman S . Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes. Cell. 1981; 24(1):125-34. DOI: 10.1016/0092-8674(81)90508-0. View

11.

Maki R, Roeder W, Traunecker A, Sidman C, Wabl M, Raschke W . The role of DNA rearrangement and alternative RNA processing in the expression of immunoglobulin delta genes. Cell. 1981; 24(2):353-65. DOI: 10.1016/0092-8674(81)90325-1. View

12.

Cami B, Bregegere F, Abastado J, Kourilsky P . Multiple sequences related to classical histocompatibility antigens in the mouse genome. Nature. 1981; 291(5817):673-5. DOI: 10.1038/291673a0. View

13.

Soloski M, UHR J, Flaherty L, Vitetta E . Qa-2, H-2K, and H-2D alloantigens evolved from a common ancestral gene. J Exp Med. 1981; 153(5):1080-93. PMC: 2186164. DOI: 10.1084/jem.153.5.1080. View

14.

Nathenson S, Uehara H, Ewenstein B, Kindt T, Coligan J . Primary structural: analysis of the transplantation antigens of the murine H-2 major histocompatibility complex. Annu Rev Biochem. 1981; 50:1025-52. DOI: 10.1146/annurev.bi.50.070181.005113. View

15.

Breathnach R, Chambon P . Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem. 1981; 50:349-83. DOI: 10.1146/annurev.bi.50.070181.002025. View

16.

Bregegere F, Abastado J, Kvist S, Rask L, Lalanne J, Garoff H . Structure of C-terminal half of two H-2 antigens from cloned mRNA. Nature. 1981; 292(5818):78-81. DOI: 10.1038/292078a0. View

17.

Steinmetz M, Moore K, Frelinger J, Sher B, Shen F, Boyse E . A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains. Cell. 1981; 25(3):683-92. DOI: 10.1016/0092-8674(81)90175-6. View

18.

Klein J, Figueroa F . Polymorphism of the mouse H-2 loci. Immunol Rev. 1981; 60:23-57. DOI: 10.1111/j.1600-065x.1981.tb00361.x. View

19.

Moore K, Sher B, Sun Y, Eakle K, Hood L . DNA sequence of a gene encoding a BALB/c mouse Ld transplantation antigen. Science. 1982; 215(4533):679-82. DOI: 10.1126/science.7058332. View

20.

Mount S . A catalogue of splice junction sequences. Nucleic Acids Res. 1982; 10(2):459-72. PMC: 326150. DOI: 10.1093/nar/10.2.459. View