Nucleotide Sequence of the PapA Gene Encoding the Pap Pilus Subunit of Human Uropathogenic Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1984 Jan 1

PMID 6140260

Citations 74

Authors

M Baga

S Normark

J Hardy

P OHanley

D Lark

O Olsson

G Schoolnik

S Falkow

Affiliations

Soon will be listed here.

Abstract

The papA gene of the uropathogenic strain Escherichia coli J96, coding for the Pap pili subunit, was subjected to DNA sequencing, and found to code for an 185-amino acid-long polypeptide with a 22-amino acid-long signal peptide. Here we present the primary sequence, the hydrophilicity profile, and the predicted polypeptide secondary structure of the Pap pili subunit.

Citing Articles

Comparative genomics of 84 Pectobacterium genomes reveals the variations related to a pathogenic lifestyle.

Li X, Ma Y, Liang S, Tian Y, Yin S, Xie S BMC Genomics. 2018; 19(1):889.

PMID: 30526490 PMC: 6286560. DOI: 10.1186/s12864-018-5269-6.

Molecular basis of usher pore gating in Escherichia coli pilus biogenesis.

Volkan E, Kalas V, Pinkner J, Dodson K, Henderson N, Pham T Proc Natl Acad Sci U S A. 2013; 110(51):20741-6.

PMID: 24297893 PMC: 3870681. DOI: 10.1073/pnas.1320528110.

The structure of the PapD-PapGII pilin complex reveals an open and flexible P5 pocket.

Ford B, Verger D, Dodson K, Volkan E, Kostakioti M, Elam J J Bacteriol. 2012; 194(23):6390-7.

PMID: 23002225 PMC: 3497488. DOI: 10.1128/JB.06651-11.

Domain activities of PapC usher reveal the mechanism of action of an Escherichia coli molecular machine.

Volkan E, Ford B, Pinkner J, Dodson K, Henderson N, Thanassi D Proc Natl Acad Sci U S A. 2012; 109(24):9563-8.

PMID: 22645361 PMC: 3386062. DOI: 10.1073/pnas.1207085109.

Structural and mechanical properties of Klebsiella pneumoniae type 3 Fimbriae.

Chen F, Chan C, Huang Y, Liu K, Peng H, Chang H J Bacteriol. 2011; 193(7):1718-25.

PMID: 21239584 PMC: 3067671. DOI: 10.1128/JB.01395-10.

References

McDonell M, Simon M, Studier F . Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977; 110(1):119-46. DOI: 10.1016/s0022-2836(77)80102-2. View

Normark S, Lark D, Hull R, Norgren M, Baga M, OHanley P . Genetics of digalactoside-binding adhesin from a uropathogenic Escherichia coli strain. Infect Immun. 1983; 41(3):942-9. PMC: 264592. DOI: 10.1128/iai.41.3.942-949.1983. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Garnier J, Osguthorpe D, Robson B . Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J Mol Biol. 1978; 120(1):97-120. DOI: 10.1016/0022-2836(78)90297-8. View

Eden C, Eriksson B, Hanson L, Jodal U, Kaijser B, Janson G . Adhesion to normal human uroepithelial cells of Escherichia coli from children with various forms of urinary tract infection. J Pediatr. 1978; 93(3):398-403. DOI: 10.1016/s0022-3476(78)81145-7. View

Evans Jr D, Evans D, DuPont H . Hemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken, and guinea pig erythrocytes in the presence and absence of mannose. Infect Immun. 1979; 23(2):336-46. PMC: 414170. DOI: 10.1128/iai.23.2.336-346.1979. View

Siebenlist U, Simpson R, Gilbert W . E. coli RNA polymerase interacts homologously with two different promoters. Cell. 1980; 20(2):269-81. DOI: 10.1016/0092-8674(80)90613-3. View

Inouye M, Halegoua S . Secretion and membrane localization of proteins in Escherichia coli. CRC Crit Rev Biochem. 1980; 7(4):339-71. DOI: 10.3109/10409238009105465. View

Kallenius G, Mollby R, Svenson S, Winberg J, Hultberg H . Identification of a carbohydrate receptor recognized by uropathogenic Escherichia coli. Infection. 1980; 8 Suppl 3:288-93. DOI: 10.1007/BF01639597. View

10.

Hagberg L, Jodal U, Korhonen T, Lindberg U, Svanborg Eden C . Adhesion, hemagglutination, and virulence of Escherichia coli causing urinary tract infections. Infect Immun. 1981; 31(2):564-70. PMC: 351345. DOI: 10.1128/iai.31.2.564-570.1981. View

11.

SANGER F, Coulson A, Barrell B, Smith A, Roe B . Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980; 143(2):161-78. DOI: 10.1016/0022-2836(80)90196-5. View

12.

Beachey E . Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface. J Infect Dis. 1981; 143(3):325-45. DOI: 10.1093/infdis/143.3.325. View

13.

Hopp T, Woods K . Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A. 1981; 78(6):3824-8. PMC: 319665. DOI: 10.1073/pnas.78.6.3824. View

14.

Kallenius G, Svenson S, Mollby R, Cedergren B, Hultberg H, Winberg J . Structure of carbohydrate part of receptor on human uroepithelial cells for pyelonephritogenic Escherichia coli. Lancet. 1981; 2(8247):604-6. DOI: 10.1016/s0140-6736(81)92743-4. View

15.

Klemm P . The complete amino-acid sequence of the K88 antigen, a fimbrial protein from Escherichia coli. Eur J Biochem. 1981; 117(3):617-27. DOI: 10.1111/j.1432-1033.1981.tb06382.x. View

16.

Hull R, GILL R, Hsu P, Minshew B, Falkow S . Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate. Infect Immun. 1981; 33(3):933-8. PMC: 350799. DOI: 10.1128/iai.33.3.933-938.1981. View

17.

Gold L, Pribnow D, Schneider T, Shinedling S, Singer B, Stormo G . Translational initiation in prokaryotes. Annu Rev Microbiol. 1981; 35:365-403. DOI: 10.1146/annurev.mi.35.100181.002053. View

18.

Bergstrom S, Olsson O, Normark S . Common evolutionary origin of chromosomal beta-lactamase genes in enterobacteria. J Bacteriol. 1982; 150(2):528-34. PMC: 216398. DOI: 10.1128/jb.150.2.528-534.1982. View

19.

Ikemura T . Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: a proposal for a synonymous codon choice that is optimal for the E. coli translational system. J Mol Biol. 1981; 151(3):389-409. DOI: 10.1016/0022-2836(81)90003-6. View

20.

Klemm P, ORSKOV I, ORSKOV F . F7 and type 1-like fimbriae from three Escherichia coli strains isolated from urinary tract infections: protein chemical and immunological aspects. Infect Immun. 1982; 36(2):462-8. PMC: 351250. DOI: 10.1128/iai.36.2.462-468.1982. View