[15N] Leucine As a Source of [15N] Glutamate in Organotypic Cerebellar Explants

Overview

Journal Biochem Biophys Res Commun

Publisher Elsevier

Specialty Biochemistry

Date 1983 Aug 30

PMID 6137216

Citations 15

Authors

M Yudkoff

I Nissim

S Kim

D Pleasure

K Hummeler

S Segal

Affiliations

Soon will be listed here.

Abstract

Approximately 26.0% of the [15N] glutamate and [alpha 15N] glutamine formed in organotypic cerebellar explants was derived from [15N] leucine. Approximately 14.0% of the 15NH3 and [amide 15N] glutamine synthesized came from leucine nitrogen. Another 4.0% of the alpha nitrogen of both glutamate and glutamine was derived from [15N] valine. These results suggest that branched-chain amino acids, particularly leucine, may be important for the synthesis of glutamic acid by the brain.

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