Cell Contact-mediated Regulation of Tyrosine Hydroxylase Synthesis in Cultured Bovine Adrenal Chromaffin Cells

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1983 Sep 1

PMID 6136522

Citations 12

Authors

A L Acheson

H Thoenen

Affiliations

Soon will be listed here.

Abstract

The specific activity of tyrosine hydroxylase (TH) in bovine adrenal chromaffin cells can be controlled by changing cell density. Chromaffin cells initially plated at low density (2-3 X 10(4) cells/cm2), and subsequently replated at a 10-fold higher density showed a sixfold increase in specific TH activity within 48 h, resulting from enhanced synthesis (increased number of TH molecules as demonstrated by immunotitration and blockade by cycloheximide) rather than activation. The density-mediated TH induction was blocked by inhibitors of both messenger RNA synthesis (alpha-amanitin) and processing (9-beta-arabinofuranosyladenine), indicating a transcriptional level of regulation. Medium conditioned by high density replated cells could not mimic the effect of high density plating itself, thus direct cell contact, rather than a diffusible factor, is responsible for the density-mediated TH induction. Since neither acetylcholinesterase nor lactate dehydrogenase specific activities were increased by high cell density, it can be concluded that the contact-mediated induction of TH is rather specific, and not the result of a general process of enzyme induction.

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References

Mueller R, Thoenen H, Axelrod J . Increase in tyrosine hydroxylase activity after reserpine administration. J Pharmacol Exp Ther. 1969; 169(1):74-9. View

Blume A, Gilbert F, Wilson S, Farber J, Rosenberg R, Nirenberg M . Regulation of acetylcholinesterase in neuroblastoma cells. Proc Natl Acad Sci U S A. 1970; 67(2):786-92. PMC: 283274. DOI: 10.1073/pnas.67.2.786. View

Chambon P . Eukaryotic nuclear RNA polymerases. Annu Rev Biochem. 1975; 44:613-38. DOI: 10.1146/annurev.bi.44.070175.003145. View

Max S, Rohrer H, Otten U, Thoenen H . Nerve growth factor-mediated induction of tyrosine hydroxylase in rat superior cervical ganglia in vitro. J Biol Chem. 1978; 253(22):8013-5. View

Hanson G, Partlow L . Stimulation of non-neuronal cell proliferation in vitro by mitogenic factors present in highly purified sympathetic neurons. Brain Res. 1978; 159(1):195-210. DOI: 10.1016/0006-8993(78)90120-8. View

Kapatos G, Zigmond M . Effect of haloperidol on dopamine synthesis and tyrosine hydroxylase in striatal synaptosomes. J Pharmacol Exp Ther. 1979; 208(3):468-75. View

Lucas C, Edgar D, Thoenen H . Regulation of tyrosine hydroxylase and choline acetyltransferase activities by cell density in the PC12 rat pheochromocytoma clonal cell line. Exp Cell Res. 1979; 121(1):79-86. DOI: 10.1016/0014-4827(79)90446-4. View

FRAZIER W, Glaser L . Surface components and cell recognition. Annu Rev Biochem. 1979; 48:491-523. DOI: 10.1146/annurev.bi.48.070179.002423. View

Renaud B, Joh T, Synder D, Reis D . Induction and delayed activation of tyrosine hydroxylase in noradrenergic neurons of A1 and A2 groups of medulla oblongata of rat. Brain Res. 1979; 176(1):169-74. DOI: 10.1016/0006-8993(79)90881-3. View

10.

Salzer J, Williams A, Glaser L, Bunge R . Studies of Schwann cell proliferation. II. Characterization of the stimulation and specificity of the response to a neurite membrane fraction. J Cell Biol. 1980; 84(3):753-66. PMC: 2110566. DOI: 10.1083/jcb.84.3.753. View

11.

Salzer J, Bunge R, Glaser L . Studies of Schwann cell proliferation. III. Evidence for the surface localization of the neurite mitogen. J Cell Biol. 1980; 84(3):767-78. PMC: 2110586. DOI: 10.1083/jcb.84.3.767. View

12.

Rieger F, Shelanski M, Greene L . The effects of nerve growth factor on acetylcholinesterase and its multiple forms in cultures of rat PC12 pheochromocytoma cells: increased total specific activity and appearance of the 16 S molecular form. Dev Biol. 1980; 76(1):238-43. DOI: 10.1016/0012-1606(80)90376-0. View

13.

BUNGE R, Glaser L, Lieberman M, Raben D, Salzer J, Whittenberger B . Growth control by cell to cell contact. J Supramol Struct. 1979; 11(2):175-87. DOI: 10.1002/jss.400110207. View

14.

Naujoks K, Korsching S, Rohrer H, Thoenen H . Nerve growth factor-mediated induction of tyrosine hydroxylase and of neurite outgrowth in cultures of bovine adrenal chromaffin cells: dependence on developmental stage. Dev Biol. 1982; 92(2):365-79. DOI: 10.1016/0012-1606(82)90182-8. View

15.

Rose K, Leonard T, Carter T . Effects of adenine nucleosides on RNA synthesis in adenovirus-infected cells. 9-beta-D-Arabinofuranosyladenine as a selective inhibitor of RNA polyadenylation. Mol Pharmacol. 1982; 22(2):517-23. View

16.

Keshishian H, Bentley D . Embryogenesis of peripheral nerve pathways in grasshopper legs. II. The major nerve routes. Dev Biol. 1983; 96(1):103-15. DOI: 10.1016/0012-1606(83)90315-9. View

17.

Keshishian H, Bentley D . Embryogenesis of peripheral nerve pathways in grasshopper legs. I. The initial nerve pathway to the CNS. Dev Biol. 1983; 96(1):89-102. DOI: 10.1016/0012-1606(83)90314-7. View

18.

Moscona A, LINSER P . Developmental and experimental changes in retinal glia cells: cell interactions and control of phenotype expression and stability. Curr Top Dev Biol. 1983; 18:155-88. DOI: 10.1016/s0070-2153(08)60582-7. View

19.

Johnson M . The intracellular distribution of glycolytic and other enzymes in rat-brain homogenates and mitochondrial preparations. Biochem J. 1960; 77:610-8. PMC: 1205083. DOI: 10.1042/bj0770610. View