Isolation and Chemical Characterization of Somatostatin-28 from Rat Hypothalamus

Overview

Journal Regul Pept

Specialty Biochemistry

Date 1981 Nov 1

PMID 6117931

Citations 3

Authors

P Bohlen

P BRAZEAU

F Esch

N Ling

R Guillemin

Affiliations

Soon will be listed here.

Abstract

A peptide with somatostatin-like immuno- and bioactivity has been isolated from an aqueous extract of 96 800 rat hypothalamic by means of immunoaffinity chromatography, gel filtration and reverse-phase high-performance liquid chromatography (HPLC). Chemical characterization by amino acid analysis, tryptic peptide mapping and retention behavior in two HPLC system showed that the peptide was indistinguishable from somatostatin-28 previously characterized from several species. This evidence suggests that rat hypothalamic somatostatin-28 is identical in structure to porcine and ovine somatostatin-28.

Citing Articles

Central somatostatin receptor 1 activation reverses acute stress-related alterations of gastric and colonic motor function in mice.

Stengel A, Goebel-Stengel M, Wang L, Larauche M, Rivier J, Tache Y Neurogastroenterol Motil. 2011; 23(6):e223-36.

PMID: 21564422 PMC: 3683311. DOI: 10.1111/j.1365-2982.2011.01706.x.

Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase.

Jolly D, Okayama H, Berg P, Esty A, Filpula D, Bohlen P Proc Natl Acad Sci U S A. 1983; 80(2):477-81.

PMID: 6300847 PMC: 393401. DOI: 10.1073/pnas.80.2.477.

Presence of somatostatin-28-(1-12) in hypothalamus and pancreas.

Benoit R, Bohlen P, Ling N, Briskin A, Esch F, BRAZEAU P Proc Natl Acad Sci U S A. 1982; 79(3):917-21.

PMID: 6121326 PMC: 345864. DOI: 10.1073/pnas.79.3.917.