Article: Stimulus-induced release of endogenous amino acids from skins containing the lateral-line organ in Xenopus laevis

The release of endogenous amino acids from skins of Xenopus laevis was studied by comparing the effects of pulsatile water motion on the efflux of glutamate and aspartate from isolated skins with and without lateral-line stitches. The stimulated efflux of glutamate from stitch skins was significantly greater than its stimulated efflux from non-stitch skins (p < 0.01). The results for aspartate were less clear cut. Although the source and mechanisms underlying the greater stimulated efflux from stitch skins remain to be determined, the results demonstrate that differences can be detected between stitch and non-stitch skins under stimulus conditions which should induce release of the afferent transmitter substance(s).

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PMID: 21276816 PMC: 5937940. DOI: 10.1016/j.pharmthera.2011.01.005.

References

1.

Weinreich D, Hammerschlag R . Nerve impulse-enhanced release of amino acids from non-synaptic regions of peripheral and central nerve trunks of bullfrog. Brain Res. 1975; 84(1):137-42. DOI: 10.1016/0006-8993(75)90807-0. View

2.

van HARREVELD A, Fifkova E . Glutamate release from the retina during spreading depression. J Neurobiol. 1970; 2(1):13-29. DOI: 10.1002/neu.480020103. View

3.

Bobbin R, Thompson M . Effects of putative transmitters on afferent cochlear transmission. Ann Otol Rhinol Laryngol. 1978; 87(2 Pt 1):185-90. DOI: 10.1177/000348947808700207. View

4.

Klinke R, Oertel W . Evidence that 5-HT is not the afferent transmitter in the cochlea. Exp Brain Res. 1977; 30(1):141-3. DOI: 10.1007/BF00237864. View

5.

Steinbach A, Bennett M . Effects of divalent ions and drugs on synaptic transmission in phasic electroreceptors in a mormyrid fish. J Gen Physiol. 1971; 58(5):580-98. PMC: 2226038. DOI: 10.1085/jgp.58.5.580. View

6.

GUTH P, Norris C, Bobbin R . The pharmacology of transmission in the peripheral auditory system. Pharmacol Rev. 1976; 28(2):95-125. View

7.

Comis S, Leng G . Action of putative neurotransmitters in the guinea pig cochlea. Exp Brain Res. 1979; 36(1):119-28. DOI: 10.1007/BF00238472. View

8.

Godfrey D, Carter J, Berger S, Matschinsky F . Levels of putative transmitter amino acids in the guinea pig cochlea. J Histochem Cytochem. 1976; 24(2):468-70. DOI: 10.1177/24.2.1254943. View

9.

WERMAN R . Criteria for identification of a central nervous system transmitter. Comp Biochem Physiol. 1966; 18(4):745-66. DOI: 10.1016/0010-406x(66)90209-x. View

10.

Bobbin R . Glutamate and aspartate mimic the afferent transmitter in the cochlea. Exp Brain Res. 1979; 34(2):389-93. DOI: 10.1007/BF00235683. View

11.

Klinke R, Evans E . Evidence that catecholamines are not the afferent transmitter in the cochlea. Exp Brain Res. 1977; 28(3-4):315-24. DOI: 10.1007/BF00235713. View

12.

Berger S, Carter J, LOWRY O . The distribution of glycine, GABA, glutamate and aspartate in rabbit spinal cord, cerebellum and hippocampus. J Neurochem. 1977; 28(1):149-58. DOI: 10.1111/j.1471-4159.1977.tb07720.x. View

13.

Klinke R, Oertel W . Evidence that GABA is not the afferent transmitter in the cochlea. Exp Brain Res. 1977; 28(3-4):311-4. DOI: 10.1007/BF00235712. View

Stimulus-induced Release of Endogenous Amino Acids from Skins Containing the Lateral-line Organ in Xenopus Laevis