Mutations in Two Unlinked Genes Are Required to Produce Asparagine Auxotrophy in Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1980 Apr 1

PMID 6102983

Citations 38

Authors

J Felton

S Michaelis

A Wright

Affiliations

Soon will be listed here.

Abstract

Escherichia coli K-12 has two genes, asnA+ and asnB+, either one of which is able to satisfy the need of cells for asparagine. In order for a strain to have an auxotrophic requirement for asparagine, both genes must be mutationally inactivated. We obtained mutants with Tn5 inserted in asnB. asnB was mapped by conjugation and by three-factor P1 transductions at 15 min on the E coli K-12 linkage map, between ubiF and nagB. Specialized transducing phage lamba 781 supE was shown to carry asnB, as well as supE, ubiF, nagA, and nagB. asnA is the previously mapped ilv-linked asn locus, whiich is between uncB and rbs. E. coli C also has two asn genes, corresponding to asnA and asnB.

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References

Signer E, Beckwith J, Brenner S . Mapping of suppressor loci in Escherichia coli. J Mol Biol. 1965; 14(1):153-66. DOI: 10.1016/s0022-2836(65)80237-6. View

White R . Control of amino sugar metabolism in Escherichia coli and isolation of mutants unable to degrade amino sugars. Biochem J. 1968; 106(4):847-58. PMC: 1198589. DOI: 10.1042/bj1060847. View

Glansdorff N, Sand G, Verhoef C . The dual genetic control of ornithine transcarbamylase synthesis in Escherichia coli K12. Mutat Res. 1967; 4(6):743-51. DOI: 10.1016/0027-5107(67)90083-8. View

Cedar H, Schwartz J . Production of L-asparaginase II by Escherichia coli. J Bacteriol. 1968; 96(6):2043-8. PMC: 252556. DOI: 10.1128/jb.96.6.2043-2048.1968. View

Cedar H, Schwartz J . The asparagine synthetase of Escherhic coli. I. Biosynthetic role of the enzyme, purification, and characterization of the reaction products. J Biol Chem. 1969; 244(15):4112-21. View

Epstein W, Davies M . Potassium-dependant mutants of Escherichia coli K-12. J Bacteriol. 1970; 101(3):836-43. PMC: 250399. DOI: 10.1128/jb.101.3.836-843.1970. View

Young I, McCann L, Stroobant P, Gibson F . Characterization and genetic analysis of mutant strains of Escherichia coli K-12 accumulating the biquinone precursors 2-octaprenyl-6-methoxy-1,4-benzoquinone and 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone. J Bacteriol. 1971; 105(3):769-78. PMC: 248499. DOI: 10.1128/jb.105.3.769-778.1971. View

Legrain C, Halleux P, Stalon V, Glansdorff N . The dual genetic control of ornithine carbamolytransferase in Escherichia coli. A case of bacterial hybrid enzymes. Eur J Biochem. 1972; 27(1):93-102. DOI: 10.1111/j.1432-1033.1972.tb01814.x. View

Low K . Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972; 36(4):587-607. PMC: 408333. DOI: 10.1128/br.36.4.587-607.1972. View

10.

Holmes R, Russell R . Mutations affecting amino sugar metabolism in Escherichia coli K-12. J Bacteriol. 1972; 111(1):290-1. PMC: 251271. DOI: 10.1128/jb.111.1.290-291.1972. View

11.

Kikuchi A, GORINI L . Similarity of genes argF and argI. Nature. 1975; 256(5519):621-4. DOI: 10.1038/256621a0. View

12.

Schrenk W, WEISBERG R . A simple method for making new transducing lines of coliphage lambda. Mol Gen Genet. 1975; 137(2):101-7. DOI: 10.1007/BF00341676. View

13.

Bachmann B, Low K, Taylor A . Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976; 40(1):116-67. PMC: 413944. DOI: 10.1128/br.40.1.116-167.1976. View

14.

Borck K, Beggs J, Brammar W, Hopkins A, Murray N . The construction in vitro of transducing derivatives of phage lambda. Mol Gen Genet. 1976; 146(2):199-207. DOI: 10.1007/BF00268089. View

15.

Yamamoto M, Nomura M, Ohsawa H, Maruo B . Identification of a temperature-sensitive asparaginyl-transfer ribonucleic acid synthetase mutant of Escherichia coli. J Bacteriol. 1977; 132(1):127-31. PMC: 221835. DOI: 10.1128/jb.132.1.127-131.1977. View

16.

FISCHER E, Wolf H, Hantke K, Parmeggiani A . Elongation factor Tu resistant to kirromycin in an Escherichia coli mutant altered in both tuf genes. Proc Natl Acad Sci U S A. 1977; 74(10):4341-5. PMC: 431937. DOI: 10.1073/pnas.74.10.4341. View

17.

Kleckner N, Roth J, Botstein D . Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics. J Mol Biol. 1977; 116(1):125-59. DOI: 10.1016/0022-2836(77)90123-1. View

18.

Guardiola J, De Felice M, Lamberti A, Iaccarino M . The acetolactate synthase isoenzymes of Escherichia coli K-12. Mol Gen Genet. 1977; 156(1):17-25. DOI: 10.1007/BF00272247. View

19.

Ikemura T, Ozeki H . Gross map location of Escherichia coli transfer RNA genes. J Mol Biol. 1977; 117(2):419-46. DOI: 10.1016/0022-2836(77)90136-x. View

20.

Jones G . L-Asparagine auxotrophs of Saccharomyces cerevisiae: genetic and phenotypic characterization. J Bacteriol. 1978; 134(1):200-7. PMC: 222235. DOI: 10.1128/jb.134.1.200-207.1978. View