Isolation and Purification of Multiple Forms of Gamma-glutamyl Transpeptidase from Rat Brain

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1980 Feb 1

PMID 6102743

Citations 9

Authors

E Reyes

T D Barela

Affiliations

Soon will be listed here.

Abstract

Four different forms of the enzyme gamma-glutamyl transpeptidase were isolated from rat brain by chromatography on concanavalin A. An approximate 1500-fold purification was achieved. The four forms were characterized with respect to molecular weight, Km for gamma-glutamyl-p-nitroanilide, mobility on polyacrylamide gels, and inhibitory effects of borate-serine. The multiple forms of the enzyme were found to have molecular weights ranging from 74,000 to 234,000 and Kms of 0.07 to 8.6 mM. It was determined that in brain, the major portion of the enzyme activity is associated with plasma membrane fragments and endoplasmic reticulum.

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References

Reyes E . Brain gamma-glutamyl transpeptidase levels in mice genetically selected for sensitivity to ethanol. Res Commun Chem Pathol Pharmacol. 1977; 17(2):353-6. View

Grandgeorge M, MORELIS P . [Partial purification and study of gamma-glutamyl transpeptidase from sheep cerebral capillaries]. Biochimie. 1976; 58(3):275-84. DOI: 10.1016/s0300-9084(76)80434-8. View

Hughey R, Curthoys N . Comparison of the size and physical properties of gamma-glutamyltranspeptidase purified from rat kidney following solubilization with papain or with Triton X-100. J Biol Chem. 1976; 251(24):8763-70. View

Tsuji M, Matsuoka Y, Nakajima T . Studies on formation of gamma-glutamylamines in rat brain and their synthetic and catabolic enzymes. J Neurochem. 1977; 29(4):633-8. DOI: 10.1111/j.1471-4159.1977.tb07779.x. View

Reyes E . Effects of alcohol on the isoenzymes of rat brain gamma-glutamyl transpeptidase. Proc West Pharmacol Soc. 1977; 20:345-9. View

Reyes E . Rat brain gamma-glutamyl transpeptidase: effects of alcohol. Res Commun Chem Pathol Pharmacol. 1978; 21(1):145-8. View

Tate S, Meister A . Identity of maleate-stimulated glutaminase with gamma-glutamyl transpeptidase in rat kidney. J Biol Chem. 1975; 250(12):4619-27. View

Robinson D, Lovenberg W, Keiser H, SJOERDSMA A . Effects of drugs on human blood platelet and plasma amine oxidase activity in vitro and in vivo. Biochem Pharmacol. 1968; 17(1):109-19. DOI: 10.1016/0006-2952(68)90163-9. View

Davis B . DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964; 121:404-27. DOI: 10.1111/j.1749-6632.1964.tb14213.x. View

10.

Andrews P . Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964; 91(2):222-33. PMC: 1202876. DOI: 10.1042/bj0910222. View

11.

Rosalki S, Tarlow D . Optimized determination of gamma-glutamyltransferase by reaction-rate analysis. Clin Chem. 1974; 20(9):1121-4. View

12.

Reyes E, Palmer G . Biochemical localization of gamma-glutamyl transpeptidase within cellular elements of the rat cerebral cortex. Res Commun Chem Pathol Pharmacol. 1976; 14(4):759-62. View

13.

Goore M, Thompson J . Gamma-glutamyl transpeptidase from kidney bean fruit. I. Purification and mechanism of action. Biochim Biophys Acta. 1967; 132(1):15-26. DOI: 10.1016/0005-2744(67)90187-8. View

14.

Schulman J, Goodman S, Mace J, PATRICK A, Tietze F, Butler E . Glutathionuria: inborn error of metabolism due to tissue deficiency of gamma-glutamyl transpeptidase. Biochem Biophys Res Commun. 1975; 65(1):68-74. DOI: 10.1016/s0006-291x(75)80062-3. View

15.

Okonkwo P, Orlowski M, Green J . Enzymes of the gamma-glutamyl cycle in the choroid plexus and brain. J Neurochem. 1974; 22(6):1053-8. DOI: 10.1111/j.1471-4159.1974.tb04336.x. View

16.

Meister A . On the enzymology of amino acid transport. Science. 1973; 180(4081):33-9. DOI: 10.1126/science.180.4081.33. View

17.

Huseby N . Multiple forms of gamma-glutamyltransferase in normal human liver, bile and serum. Biochim Biophys Acta. 1978; 522(2):354-62. DOI: 10.1016/0005-2744(78)90069-4. View

18.

. Activity of gamma-glutamyl transpeptidase in serum of patients receiving anticonvulsant of anticoagulant therapy. Clin Biochem. 1976; 9(2):67-70. DOI: 10.1016/s0009-9120(76)80019-7. View

19.

Heipertz R, Eickhoff K, Poser W . Anticonvulsant therapy and serum gamma-glutamyl-transferase. Klin Wochenschr. 1978; 56(18):921-8. DOI: 10.1007/BF01489219. View

20.

Miller S, Awasthi Y, Srivastava S . Studies of human kidney gamma-glutamyl transpeptidase. Purification and structural, kinetic and immunological properties. J Biol Chem. 1976; 251(8):2271-8. View