Subunit IV of Yeast Cytochrome C Oxidase: Cloning and Nucleotide Sequencing of the Gene and Partial Amino Acid Sequencing of the Mature Protein

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1984 Dec 1

PMID 6098449

Citations 48

Authors

A C Maarse

A P van Loon

H Riezman

I Gregor

G Schatz

L A Grivell

Affiliations

Soon will be listed here.

Abstract

The six small subunits (IV-VII, VIIa, VIII) of yeast cytochrome c oxidase are encoded by nuclear genes and imported into the mitochondria. We have isolated the gene for subunit IV from a yeast genomic clone bank and determined its complete nucleotide sequence. We have also isolated subunit IV from purified yeast cytochrome c oxidase and determined most of its amino acid sequence which confirms the positioning of approximately 90% of the amino acid residues. The sequence comparison shows that the coding sequence of the gene lacks introns and that subunit IV is made as a precursor with an amino-terminal extension of 25 residues, five of which are basic and none of them acidic. Precursor processing involves cleavage of a Leu-Gln bond.

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