Escherichia Coli Alpha-haemolysin Synthesis and Export Genes Are Flanked by a Direct Repetition of IS91-like Elements

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1984 Jan 1

PMID 6096676

Citations 21

Authors

J C Zabala

J M Garcia-Lobo

F de la Cruz

J M Ortiz

Affiliations

Soon will be listed here.

Abstract

A revised physical map of the alpha-haemolysin plasmid pHly152 has been constructed. The known position of the hly genes in the restriction map of pHly152 allowed us to locate in it a direct repeat of IS elements flanking the hly genes of pHly152. These elements are IS92L, which is a derivative of the previously characterised element IS91 (1.85 kb) by insertion of a sequence of 1.2 kb, and IS92R, an element related to IS91 by a deletion of 0.7 kb and substitution of a 0.2 kb sequence of IS91 by a 1.2 kb heterologous sequence. IS92L is, in turn, flanked by an inverted repetition of sequences of 1.4 kb. These and previously published data strongly suggest that the hly genes spread at some time in evolution by means of the recombinational activity of IS91-like elements.

Citing Articles

Antibiotics shaping bacterial genome: deletion of an IS91 flanked virulence determinant upon exposure to subinhibitory antibiotic concentrations.

Pedro L, Banos R, Aznar S, Madrid C, Balsalobre C, Juarez A PLoS One. 2011; 6(11):e27606.

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ISCR elements: novel gene-capturing systems of the 21st century?.

Toleman M, Bennett P, Walsh T Microbiol Mol Biol Rev. 2006; 70(2):296-316.

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Nested deletions of the SRL pathogenicity island of Shigella flexneri 2a.

Turner S, Luck S, Sakellaris H, Rajakumar K, Adler B J Bacteriol. 2001; 183(19):5535-43.

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Genetic rearrangements of the regions adjacent to genes encoding heat-labile enterotoxins (eltAB) of enterotoxigenic Escherichia coli strains.

Schlor S, Riedl S, Blass J, Reidl J Appl Environ Microbiol. 2000; 66(1):352-8.

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Insertion sequences.

Mahillon J, Chandler M Microbiol Mol Biol Rev. 1998; 62(3):725-74.

PMID: 9729608 PMC: 98933. DOI: 10.1128/MMBR.62.3.725-774.1998.

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