Enhanced Recombination Between Lambda Plac5 and F42lac: Identification of Cis- and Trans-acting Factors

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 Dec 1

PMID 6095300

Citations 18

Authors

H S Seifert

R D Porter

Affiliations

Soon will be listed here.

Abstract

Enhanced transductional recombination between specialized transducing phage lambda plac5 and plasmid F42lac depends on cis- and trans-acting factors. By constructing a series of recombinant molecules, the cis-acting site required for enhanced recombination has been identified as oriT, the origin of conjugational transfer of the F sex factor of Escherichia coli. The trans-acting factors are located in the promoter-proximal and the promoter-distal regions of the traY-to-traZ operon.

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