Rat Liver Asialoglycoprotein Receptor Lacks a Cleavable NH2-terminal Signal Sequence

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 Dec 1

PMID 6095287

Citations 40

Authors

E C Holland

J O Leung

K Drickamer

Affiliations

Soon will be listed here.

Abstract

Two cDNA clones encoding the predominant form of the asialoglycoprotein receptor from rat liver (the major rat hepatic lectin; RHL-1) were identified by screening a rat liver cDNA library with a mixed oligonucleotide probe 35 nucleotides long. One clone was a nearly full-length copy of the mRNA for RHL-1, while the other was shortened at both ends. The sequences of these clones demonstrate that this transmembrane receptor is not synthesized with an NH2-terminal signal sequence. The only proteolytic processing occurring in the biosynthesis of RHL-1 is the removal of the NH2-terminal initiator methionine residue. Insertion of RHL-1 into the membrane is postulated to occur by the recognition of the internal transmembrane region as a signal sequence.

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References

Uhlenbeck O, Martin F, Doty P . Self-complementary oligoribonucleotides: effects of helix defects and guanylic acid-cytidylic acid base pairs. J Mol Biol. 1971; 57(2):217-29. DOI: 10.1016/0022-2836(71)90342-1. View

Battula N, Loeb L . The infidelity of avian myeloblastosis virus deoxyribonucleic acid polymerase in polynucleotide replication. J Biol Chem. 1974; 249(13):4086-93. View

Hudgin R, PRICER Jr W, ASHWELL G, Stockert R, Morell A . The isolation and properties of a rabbit liver binding protein specific for asialoglycoproteins. J Biol Chem. 1974; 249(17):5536-43. View

Blobel G, Dobberstein B . Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J Cell Biol. 1975; 67(3):835-51. PMC: 2111658. DOI: 10.1083/jcb.67.3.835. View

Grunstein M, Hogness D . Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975; 72(10):3961-5. PMC: 433117. DOI: 10.1073/pnas.72.10.3961. View

Proudfoot N, Brownlee G . 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976; 263(5574):211-4. DOI: 10.1038/263211a0. View

Rothman J, Lenard J . Membrane asymmetry. Science. 1977; 195(4280):743-53. DOI: 10.1126/science.402030. View

Wickens M, Buell G, Schimke R . Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I. J Biol Chem. 1978; 253(7):2483-95. View

Lingappa V, Katz F, Lodish H, Blobel G . A signal sequence for the insertion of a transmembrane glycoprotein. Similarities to the signals of secretory proteins in primary structure and function. J Biol Chem. 1978; 253(24):8667-70. View

10.

Chirgwin J, Przybyla A, MacDonald R, Rutter W . Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979; 18(24):5294-9. DOI: 10.1021/bi00591a005. View

11.

Nelson T, Brutlag D . Addition of homopolymers to the 3'-ends of duplex DNA with terminal transferase. Methods Enzymol. 1979; 68:41-50. DOI: 10.1016/0076-6879(79)68005-9. View

12.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

13.

McCune J, Lingappa V, Fu S, Blobel G, KUNKEL H . Biogenesis of membrane-bound and secreted immunoglobulins. I. Two distinct translation products of human mu-chain, with identical N-termini and different C-termini. J Exp Med. 1980; 152(2):463-8. PMC: 2185946. DOI: 10.1084/jem.152.2.463. View

14.

Walter P, Blobel G . Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum. Proc Natl Acad Sci U S A. 1980; 77(12):7112-6. PMC: 350451. DOI: 10.1073/pnas.77.12.7112. View

15.

Drickamer K . Complete amino acid sequence of a membrane receptor for glycoproteins. Sequence of the chicken hepatic lectin. J Biol Chem. 1981; 256(11):5827-39. View

16.

Kozak M . Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res. 1981; 9(20):5233-52. PMC: 327517. DOI: 10.1093/nar/9.20.5233. View

17.

Sabatini D, Kreibich G, Morimoto T, Adesnik M . Mechanisms for the incorporation of proteins in membranes and organelles. J Cell Biol. 1982; 92(1):1-22. PMC: 2112015. DOI: 10.1083/jcb.92.1.1. View

18.

ASHWELL G, Harford J . Carbohydrate-specific receptors of the liver. Annu Rev Biochem. 1982; 51:531-54. DOI: 10.1146/annurev.bi.51.070182.002531. View

19.

Drickamer K, Mamon J . Phosphorylation of a membrane receptor for glycoproteins. Possible transmembrane orientation of the chicken hepatic lectin. J Biol Chem. 1982; 257(24):15156-61. View

20.

Vieira J, Messing J . The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982; 19(3):259-68. DOI: 10.1016/0378-1119(82)90015-4. View