Comparative Nucleotide Sequences Encoding the Immunity Proteins and the Carboxyl-terminal Peptides of Colicins E2 and E3

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1984 Nov 26

PMID 6095211

Citations 11

Authors

P C Lau

R W Rowsome

M Zuker

L P Visentin

Affiliations

Soon will be listed here.

Abstract

Using the M13 dideoxy sequencing technique, we have established the DNA sequences of colicins E2 and E3 which encompass the receptor-binding and the catalytic domains of each of the nucleases, and their immunity (imm) genes. The imm gene of plasmid ColE2-P9 is 255 bp long and is separated from the end of the col gene by a dinucleotide. This gene pair is arranged similarly in plasmid ColE3-CA38 except that the intergenic space is 9 bp and the E3 imm gene is one codon shorter than its E2 counterpart. Comparisons of the E2 and E3 imm sequences indicate considerable divergence whereas the receptor-binding domains of both colicins are highly conserved. The two nuclease domains appear to share some sequence homology. A possible evolutionary relationship between colicin E3 and other microbial extracellular ribonucleases is also suggested from the sequence alignment analysis.

Citing Articles

Posttranscriptional repression of the cel gene of the ColE7 operon by the RNA-binding protein CsrA of Escherichia coli.

Yang T, Sung Y, Lei G, Romeo T, Chak K Nucleic Acids Res. 2010; 38(12):3936-51.

PMID: 20378712 PMC: 2896534. DOI: 10.1093/nar/gkq177.

Molecular structures and functions of pyocins S1 and S2 in Pseudomonas aeruginosa.

Sano Y, Matsui H, Kobayashi M, Kageyama M J Bacteriol. 1993; 175(10):2907-16.

PMID: 8491711 PMC: 204608. DOI: 10.1128/jb.175.10.2907-2916.1993.

The inherent DNase of pyocin AP41 causes breakdown of chromosomal DNA.

Sano Y J Bacteriol. 1993; 175(3):912-5.

PMID: 8423163 PMC: 196246. DOI: 10.1128/jb.175.3.912-915.1993.

A novel transposon-like structure carries the genes for pyocin AP41, a Pseudomonas aeruginosa bacteriocin with a DNase domain homology to E2 group colicins.

Sano Y, Kageyama M Mol Gen Genet. 1993; 237(1-2):161-70.

PMID: 8384291 DOI: 10.1007/BF00282797.

Nucleotide sequences from the colicin E8 operon: homology with plasmid ColE2-P9.

Uchimura T, Lau P Mol Gen Genet. 1987; 209(3):489-93.

PMID: 3323826 DOI: 10.1007/BF00331154.

References

Bowman C, Dahlberg J, Ikemura T, Konisky J, Nomura M . Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo. Proc Natl Acad Sci U S A. 1971; 68(5):964-8. PMC: 389091. DOI: 10.1073/pnas.68.5.964. View

Inselburg J . Colicin factor DNA: a single non-homologous region in Col E2-E3 heteroduplex molecules. Nat New Biol. 1973; 241(112):234-7. DOI: 10.1038/newbio241234a0. View

Di Masi D, White J, Schnaitman C, Bradbeer C . Transport of vitamin B12 in Escherichia coli: common receptor sites for vitamin B12 and the E colicins on the outer membrane of the cell envelope. J Bacteriol. 1973; 115(2):506-13. PMC: 246277. DOI: 10.1128/jb.115.2.506-513.1973. View

de Graaf F, Niekus H, Klootwijk J . Inactivation of bacterial ribosomes in vivo and in vitro by cloacin DF13. FEBS Lett. 1973; 35(1):161-5. DOI: 10.1016/0014-5793(73)80601-5. View

Jakes K, Zinder N, Boon T . Purification and properties of colicin E3 immunity protein. J Biol Chem. 1974; 249(2):438-44. View

Oudega B, de Graaf F . Mode of action of the cloacin DF13-immunity protein. Biochim Biophys Acta. 1975; 392(1):184-95. View

Kool A, Pols C, Nijkamp H . Bacteriocinogenic Clo DF13 minicells of Escherichia coli synthesize a protein that accounts for immunity to bacteriocin Clo DF13: purification and characterization of the immunity protein. Antimicrob Agents Chemother. 1975; 8(1):67-75. PMC: 429263. DOI: 10.1128/AAC.8.1.67. View

Tyler J, Sherratt D . Synthesis of E colicins in Escherichia coli. Mol Gen Genet. 1975; 140(4):349-53. DOI: 10.1007/BF00267325. View

Schaller K, Nomura M . Colicin E2 is DNA endonuclease. Proc Natl Acad Sci U S A. 1976; 73(11):3989-93. PMC: 431295. DOI: 10.1073/pnas.73.11.3989. View

10.

Ohno S, Ohno-Iwashita Y, Suzuki K, Imahori K . Purification and characterization of active component and active fragment of colicin E3. J Biochem. 1977; 82(4):1045-53. DOI: 10.1093/oxfordjournals.jbchem.a131775. View

11.

Yamamoto H, Nishida K, Beppu T, Arima K . Tryptic digestion of colicin E2 and its active fragment. J Biochem. 1978; 83(3):827-34. DOI: 10.1093/oxfordjournals.jbchem.a131979. View

12.

Chou P, Fasman G . Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol Relat Areas Mol Biol. 1978; 47:45-148. DOI: 10.1002/9780470122921.ch2. View

13.

Suzuki K, Imahori K . Preparation and characterization of an active fragment of colicin E3. J Biochem. 1978; 84(5):1021-9. DOI: 10.1093/oxfordjournals.jbchem.a132216. View

14.

Suzuki K, Imahori K . Amino acid sequence of an active fragment (T2A) of colicin E3. J Biochem. 1978; 84(5):1031-9. DOI: 10.1093/oxfordjournals.jbchem.a132217. View

15.

Ohno S, Imahori K . Colicin E3 is an endonuclease. J Biochem. 1978; 84(6):1637-40. DOI: 10.1093/oxfordjournals.jbchem.a132291. View

16.

Ohno-Iwashita Y, Imahori K . Comparative studies on the structures of colicins E2 and E3. FEBS Lett. 1979; 100(2):249-52. DOI: 10.1016/0014-5793(79)80344-0. View

17.

Atkins J . Is UAA or UGA part of the recognition signal for ribosomal initiation?. Nucleic Acids Res. 1979; 7(4):1035-41. PMC: 342280. DOI: 10.1093/nar/7.4.1035. View

18.

Ohno-Iwashita Y, Imahori K . Assignment of the functional loci in colicin E2 and E3 molecules by the characterization of their proteolytic fragments. Biochemistry. 1980; 19(4):652-9. DOI: 10.1021/bi00545a008. View

19.

Rosenberg M, Court D . Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979; 13:319-53. DOI: 10.1146/annurev.ge.13.120179.001535. View

20.

Suzuki K, Ohno S, Imahori K . Studies on the physicochemical structure and stability of an active fragment (T2A) of colicin E3. J Biochem. 1980; 87(3):761-9. DOI: 10.1093/oxfordjournals.jbchem.a132805. View