A Cryptogram for Recording Rotavirus Strains: the Rotacode

Overview

Journal J Hyg (Lond)

Publisher Cambridge University Press

Specialty Public Health

Date 1984 Oct 1

PMID 6094663

Citations 3

Authors

R B Moosai

R Alcock

C R Madeley

Affiliations

Soon will be listed here.

Abstract

The RNA genome of rotaviruses consists of 11 segments in four size-classes which can be separated by polyacrylamide gel electrophoresis, although 11 separate bands are not shown by all strains. We propose a cryptogram (Rotacode) based on the relative distance of migration of adjacent bands in each size-class for coding the typical pattern of each strain of virus. This provides a shorthand for recording details of each strain and for grouping electrophoretically similar strains. Rotacode was found to be reliable and reproducible, with identical codes being obtained for the same samples in repeated experiments under code and by various observers. Rotacode was also used to analyse 189 strains obtained over a three-year period and differentiated 13 electrophoretypes. This confirms the considerable electrophoretic variability of wild strains.

Citing Articles

Detection of rotavirus by a latex agglutination test, Rotalex: comparison with electron microscopy, immunofluorescence, polyacrylamide gel electrophoresis, and enzyme linked immunosorbent assay.

Moosai R, Alcock R, Bell T, Laidler F, Peiris J, Wyn-Jones A J Clin Pathol. 1985; 38(6):694-700.

PMID: 2989342 PMC: 499270. DOI: 10.1136/jcp.38.6.694.

Longitudinal study of rotavirus infections among children from Belém, Brazil.

Linhares A, Gabbay Y, Freitas R, da Rosa E, Mascarenhas J, Loureiro E Epidemiol Infect. 1989; 102(1):129-45.

PMID: 2537221 PMC: 2249309. DOI: 10.1017/s0950268800029769.

Culture adaptation and characterization of group A rotaviruses causing diarrheal illnesses in Bangladesh from 1985 to 1986.

Ward R, Clemens J, Sack D, Knowlton D, McNeal M, Huda N J Clin Microbiol. 1991; 29(9):1915-23.

PMID: 1663517 PMC: 270235. DOI: 10.1128/jcm.29.9.1915-1923.1991.

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