Genetic Inactivation of Topoisomerase I Suppresses a Defect in Initiation of Chromosome Replication in Escherichia Coli

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1984 Jan 1

PMID 6092846

Citations 22

Authors

J Louarn

J P Bouche

J PATTE

J M Louarn

Affiliations

Soon will be listed here.

Abstract

A strain of Escherichia coli K12 harboring simultaneously the temperature-sensitive dnaA46 mutation and a deletion of the trp-topA-cysB region plates with the same full efficiency at 30 degrees C and 42 degrees C. We have analyzed the possible involvement of the gene coding for topoisomerase I, topA, in this suppression phenomenon. The Ts phenotype was retrieved upon introduction of a plasmid-borne DNA fragment including an active topA gene into this strain, but not upon introduction of the same fragment harboring a topA::Tn1000 insertion. Replication seems to remain DnaA-dependent in the delta (topA) strain, however, since we have been unable to introduce a dnaA::Tn10 allele. We propose either that the dnaA46 gene product is overproduced and compensates for its thermal inactivation, or that initiation at oriC demands less DnaA protein in the absence of topoisomerase I.

Citing Articles

Supercoiling, R-loops, Replication and the Functions of Bacterial Type 1A Topoisomerases.

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The Stringent Response Inhibits DNA Replication Initiation in E. coli by Modulating Supercoiling of .

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DNA gyrase activity regulates DnaA-dependent replication initiation in Bacillus subtilis.

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Suppression of the Escherichia coli dnaA46 mutation by changes in the activities of the pyruvate-acetate node links DNA replication regulation to central carbon metabolism.

Tymecka-Mulik J, Boss L, Maciag-Dorszynska M, Matias Rodrigues J, Gaffke L, Wosinski A PLoS One. 2017; 12(4):e0176050.

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Multiple DNA Binding Proteins Contribute to Timing of Chromosome Replication in E. coli.

Riber L, Frimodt-Moller J, Charbon G, Lobner-Olesen A Front Mol Biosci. 2016; 3:29.

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