Cellular Localization of C-fps Gene Product NCP98

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1984 Dec 1

PMID 6092720

Citations 11

Authors

J C Young

G S Martin

Affiliations

Soon will be listed here.

Abstract

We compared the intracellular location of the product of the c-fps proto-oncogene, NCP98, with that of its viral homolog P140, the transforming protein of Fujinami sarcoma virus. Using the technique of biochemical subcellular fractionation, we determined that 60 to 90% of NCP98 and its associated kinase activity are in the soluble fraction of a chicken myeloblast cell line. This fractionation behavior differs from that of P140, which is found predominantly in the particulate fraction, both in Fujinami sarcoma virus-infected chicken embryo fibroblasts and in Fujinami sarcoma virus-infected myeloblasts. The fractionation behavior of NCP98 is, however, similar to that of the P140 encoded by a temperature-sensitive strain of Fujinami sarcoma virus in infected cells grown at the nonpermissive temperature. The absence of gag sequences from NCP98 is not responsible for the difference in fractionation behavior: the v-fps transforming protein of strain F36, P91, which lacks gag sequences, is also predominantly particulate. These results indicate that association with cellular structural components correlates with the transforming activity of proteins containing fps sequences.

Citing Articles

The human c-fps/fes gene product expressed ectopically in rat fibroblasts is nontransforming and has restrained protein-tyrosine kinase activity.

Greer P, Pawson T Mol Cell Biol. 1988; 8(2):578-87.

PMID: 3352601 PMC: 363183. DOI: 10.1128/mcb.8.2.578-587.1988.

Enzymatic activation of Fujinami sarcoma virus gag-fps transforming proteins by autophosphorylation at tyrosine.

Nelson R, Branton P, Pawson T EMBO J. 1987; 6(3):659-66.

PMID: 3034604 PMC: 553448. DOI: 10.1002/j.1460-2075.1987.tb04805.x.

The first seven amino acids encoded by the v-src oncogene act as a myristylation signal: lysine 7 is a critical determinant.

Kaplan J, Mardon G, Bishop J, Varmus H Mol Cell Biol. 1988; 8(6):2435-41.

PMID: 2841581 PMC: 363442. DOI: 10.1128/mcb.8.6.2435-2441.1988.

v-rel oncoproteins in the nucleus and in the cytoplasm transform chicken spleen cells.

Gilmore T, Temin H J Virol. 1988; 62(3):703-14.

PMID: 2828665 PMC: 253623. DOI: 10.1128/JVI.62.3.703-714.1988.

The myristylation signal of p60v-src functionally complements the N-terminal fps-specific region of P130gag-fps.

Brooks-Wilson A, Ball E, Pawson T Mol Cell Biol. 1989; 9(5):2214-9.

PMID: 2747647 PMC: 363016. DOI: 10.1128/mcb.9.5.2214-2219.1989.

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