Transcription of Ribosomal Component Genes and Lac in a RelA+/relA Pair of Escherichia Coli Strains

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1984 Sep 1

PMID 6090395

Citations 8

Authors

R Little

H Bremer

Affiliations

Soon will be listed here.

Abstract

To determine the stringent response, a repression of gene activity during amino acid starvation assumed to be mediated by the effector necleotide guanosine tetraphosphate (ppGpp), of metabolically regulated constitutive genes, we measured the transcription of ribosomal protein genes, the constitutive lac operon, and stable RNA genes in a variety of growth media and after amino acid starvation in a relA+/relA pair of Escherichia coli B/r strains. For rRNA and tRNA (stable RNA) it has previously been shown that the distinction between stringent control and growth rate control is unfounded, as the function describing the stable RNA gene activities at different concentrations of guanosine tetraphosphate is independent of growth conditions (exponential growth or amino acid starvation) and of the relA allele present. Here, the results indicated that the stringent responses of ribosomal protein genes and lac differ from their metabolic control during exponential growth in different media. This can be explained by polarity and RNA polymerase sink effects during amino acid starvation which are irrelevant for stable RNA genes but which are superimposed on mRNA gene activities.

Citing Articles

The secondary messenger ppGpp interferes with cAMP-CRP regulon by promoting CRP acetylation in Escherichia coli.

Ro C, Cashel M, Fernandez-Coll L PLoS One. 2021; 16(10):e0259067.

PMID: 34705884 PMC: 8550359. DOI: 10.1371/journal.pone.0259067.

Global responses of Methanococcus maripaludis to specific nutrient limitations and growth rate.

Hendrickson E, Liu Y, Rosas-Sandoval G, Porat I, Soll D, Whitman W J Bacteriol. 2008; 190(6):2198-205.

PMID: 18203827 PMC: 2258866. DOI: 10.1128/JB.01805-07.

mRNA composition and control of bacterial gene expression.

Liang S, Xu Y, Dennis P, Bremer H J Bacteriol. 2000; 182(11):3037-44.

PMID: 10809680 PMC: 94487. DOI: 10.1128/JB.182.11.3037-3044.2000.

Expression of lacZ from the promoter of the Escherichia coli spc operon cloned into vectors carrying the W205 trp-lac fusion.

Liang S, Dennis P, Bremer H J Bacteriol. 1998; 180(23):6090-100.

PMID: 9829916 PMC: 107692. DOI: 10.1128/JB.180.23.6090-6100.1998.

Chlorophyll a availability affects psbA translation and D1 precursor processing in vivo in Synechocystis sp. PCC 6803.

He Q, Vermaas W Proc Natl Acad Sci U S A. 1998; 95(10):5830-5.

PMID: 9576970 PMC: 20465. DOI: 10.1073/pnas.95.10.5830.

References

Haseltine W, Block R . Synthesis of guanosine tetra- and pentaphosphate requires the presence of a codon-specific, uncharged transfer ribonucleic acid in the acceptor site of ribosomes. Proc Natl Acad Sci U S A. 1973; 70(5):1564-8. PMC: 433543. DOI: 10.1073/pnas.70.5.1564. View

Dennis P, Bremer H . Macromolecular composition during steady-state growth of Escherichia coli B-r. J Bacteriol. 1974; 119(1):270-81. PMC: 245599. DOI: 10.1128/jb.119.1.270-281.1974. View

Dennis P, Nomura M . Stringent control of ribosomal protein gene expression in Escherichia coli. Proc Natl Acad Sci U S A. 1974; 71(10):3819-23. PMC: 434275. DOI: 10.1073/pnas.71.10.3819. View

van Ooyen A, DE BOER H, Ab G, Gruber M . Specific inhibition of ribosomal RNA synthesis in vitro by guanosine 3' diphosphate, 5' diphosphate. Nature. 1975; 254(5500):530-1. DOI: 10.1038/254530a0. View

Dennis P, Nomura M . Stringent control of the transcriptional activities of ribosomal protein genes in E. coli. Nature. 1975; 255(5508):460-5. DOI: 10.1038/255460a0. View

Dennis P, Nomura M . Regulation of the expression of ribosomal protein genes in Escherichia coli. J Mol Biol. 1975; 97(1):61-76. DOI: 10.1016/s0022-2836(75)80022-2. View

Dalbow D, Bremer H . Metabolic regulation of beta-galactosidase synthesis in Escherichia coli. A test for constitutive ribosome synthesis. Biochem J. 1975; 150(1):1-8. PMC: 1165696. DOI: 10.1042/bj1500001b. View

Morse D, Guertin M . Regulation of mRNA utilization and degradation by amino-acid starvation. Nat New Biol. 1971; 232(2):165-9. DOI: 10.1038/newbio232165a0. View

Imamoto F, Kano Y . Inhibition of transcription of the tryptophan operon in Escherichia coli by a block in initiation of translation. Nat New Biol. 1971; 232(2):169-73. DOI: 10.1038/newbio232169a0. View

10.

Lazzarini R, CASHEL M, Gallant J . On the regulation of guanosine tetraphosphate levels in stringent and relaxed strains of Escherichia coli. J Biol Chem. 1971; 246(14):4381-5. View

11.

Rose J, Yanofsky C . Metabolic regulation of the tryptophan operon of Escherichia coli: repressor-independent regulation of transcription initiation frequency. J Mol Biol. 1972; 69(1):103-18. DOI: 10.1016/0022-2836(72)90026-5. View

12.

Dennis P, Nordan D . Characterization of the hybridization between purified 16S and 23S ribosomal ribonucleic acid and ribosomal deoxyribonucleic acid from Escherichia coli. J Bacteriol. 1976; 128(1):28-34. PMC: 232822. DOI: 10.1128/jb.128.1.28-34.1976. View

13.

Dennis P . Influence of the stringent control system on the transcription of ribosomal ribonucleic acid and ribosomal protein genes in Escherichia coli. J Bacteriol. 1977; 129(2):580-8. PMC: 234980. DOI: 10.1128/jb.129.2.580-588.1977. View

14.

Blumenthal R, Lemaux P, Neidhardt F, Dennis P . The effects of the relA gene on the synthesis of aminoacyl-tRNA synthetases and other transcription and translation proteins in Escherichia coli A. Mol Gen Genet. 1976; 149(3):291-6. DOI: 10.1007/BF00268530. View

15.

Seeburg P, Nusslein C, Schaller H . Interaction of RNA polymerase with promoters from bacteriophage fd. Eur J Biochem. 1977; 74(1):107-13. DOI: 10.1111/j.1432-1033.1977.tb11372.x. View

16.

Wanner B, Kodaira R, Neidhardt F . Physiological regulation of a decontrolled lac operon. J Bacteriol. 1977; 130(1):212-22. PMC: 235196. DOI: 10.1128/jb.130.1.212-222.1977. View

17.

Lindahl L, Post L, Zengel J, Gilbert S, Strycharz W, Nomura M . Mapping of ribosomal protein genes by in vitro protein synthesis using DNA fragments of lambdafus3 transducing phage DNA as templates. J Biol Chem. 1977; 252(20):7365-83. View

18.

Maher D, Dennis P . In vivo transcription of E. coli genes coding for rRNA, ribosomal proteins and subunits of RNA polymerase: influence of the stringent control system. Mol Gen Genet. 1977; 155(2):203-11. DOI: 10.1007/BF00393161. View

19.

Gausing K . Regulation of ribosome production in Escherichia coli: synthesis and stability of ribosomal RNA and of ribosomal protein messenger RNA at different growth rates. J Mol Biol. 1977; 115(3):335-54. DOI: 10.1016/0022-2836(77)90158-9. View

20.

Dennis P . Transcription patterns of adjacent segments on the chromosome of Escherichia coli containing genes coding for four 50S ribosomal proteins and the beta and beta' subunits of RNA polymerase. J Mol Biol. 1977; 115(4):603-25. DOI: 10.1016/0022-2836(77)90105-x. View