Monoclonal Antibody to Simian Virus 40 Small T

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1984 Sep 1

PMID 6088798

Citations 13

Authors

X Montano

D P Lane

Affiliations

Soon will be listed here.

Abstract

A monoclonal antibody, PAb280, was produced that recognizes simian virus 40 (SV40) small t but does not react with SV40 large T. The specificity of the antibody was analyzed by immunoprecipitation of labeled cell extracts, Western blotting, and immunocytochemistry. Small t was found to accumulate late in the SV40 lytic cycle and was localized in both the cytoplasm and the nucleus of cells infected with wild-type SV40. Importantly, antibodies against determinants common to SV40 large T and small t did not appear to be able to recognize the cytoplasmic form of SV40 small t at the immunocytochemical level. The localization of small t within the nucleus appeared to be distinct from that of large T.

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