Cell-specified Expression of a Selectable Hybrid Gene

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 Aug 1

PMID 6087339

Citations 27

Authors

V Episkopou

A J Murphy

A Efstratiadis

Affiliations

Soon will be listed here.

Abstract

We have inserted into a retroviral vector the structural information encoding the selectable bacterial genes neo and gpt linked, respectively, to the promoters of the herpesvirus thymidine kinase gene and the rat preproinsulin II gene. We have used this recombinant retrovirus construction to transduce insulinoma cell lines that are positive or negative for insulin expression. Selection of the transductants for neo yielded resistant colonies from all of the cell lines, while selection for gpt yielded resistant transductants in relatively high frequency only from insulin-producing cells. The 5' end of the gpt transcripts maps to the authentic preproinsulin capping site of the construction.

Citing Articles

Developmental and tissue-specific expression of a family of transcripts related to rat insulin-like growth factor II mRNA.

Soares M, Ishii D, Efstratiadis A Nucleic Acids Res. 1985; 13(4):1119-34.

PMID: 3889836 PMC: 341060. DOI: 10.1093/nar/13.4.1119.

A cell type specific factor recognizes the rat thyroglobulin promoter.

Musti A, Ursini V, Avvedimento E, Zimarino V, Di Lauro R Nucleic Acids Res. 1987; 15(20):8149-66.

PMID: 3671079 PMC: 306350. DOI: 10.1093/nar/15.20.8149.

Human globin gene promoter sequences are sufficient for specific expression of a hybrid gene transfected into tissue culture cells.

Rutherford T, Nienhuis A Mol Cell Biol. 1987; 7(1):398-402.

PMID: 3561396 PMC: 365081. DOI: 10.1128/mcb.7.1.398-402.1987.

The role of somatic cell genetics in human gene mapping.

Grzeschik K Experientia. 1986; 42(10):1128-37.

PMID: 3533605 DOI: 10.1007/BF01941287.

Cloning vectors for expression of cDNA libraries in mammalian cells.

Murphy A, Efstratiadis A Proc Natl Acad Sci U S A. 1987; 84(23):8277-81.

PMID: 3479791 PMC: 299525. DOI: 10.1073/pnas.84.23.8277.

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