The Calcium-stimulated Incorporation of Ethanolamine and Serine into the Phospholipids of the Housefly Musca Domestica

Overview

Journal Biochem J

Specialty Biochemistry

Date 1967 Aug 1

PMID 6048809

Citations 5

Authors

H D Crone

Affiliations

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Abstract

1. The calcium-stimulated incorporation of [2-(14)C]ethanolamine and l-[3-(14)C]-serine into the phospholipids of homogenates of the fat bodies from larval houseflies (Musca domestica) was studied. 2. Ethanolamine and serine acted as competitive inhibitors with one another. N-Methylethanolamine was not distinguished from ethanolamine by the system. Tris buffer was also a competitor with these compounds, and a number of other amino alcohols were inhibitory, probably competitively. 3. The incorporation of [(32)P]phosphorylethanolamine into phospholipids was observed in suspensions of whole fat bodies. This incorporation was stimulated by magnesium. 4. During the incubation of the homogenates, a calcium-stimulated breakdown of phospholipids by a phospholipase A occurred. 5. These results are compared with results published for similar mammalian systems, and their possible physiological significance is discussed.

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