A New Method for Determination of Inorganic Phosphorus in Serum Without Deproteinization

Overview

Journal J Clin Chem Clin Biochem

Specialty Biochemistry

Date 1977 Dec 1

PMID 604424

Citations 11

Authors

M M El-Merzabani

N I Zakhary

Affiliations

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Abstract

A simple method was developed for the determination of inorganic phosphorus in serum without deproteinization. The method is based on the use of formic acid as protein solubilizer and glycerol as stabilizer for the assay system. The optimal conditions for colour development were determined. The results obtained with the new method correlate well with those obtained after deproteinization of serum with trichloroacetic acid. The present method could be fully mechanized, and its application to the determination of serum phosphatases is discussed.

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