Species Differences in the Inhibition of Glutathione S-aryltransferase by Phthaleins and Dicarboxylic Acids

Overview

Journal Biochem J

Specialty Biochemistry

Date 1967 Apr 1

PMID 6033774

Citations 15

Authors

A G Clark

F J DARBY

J N Smith

Affiliations

Soon will be listed here.

Abstract

1. Glutathione-S-aryltransferase activity from grass grubs (Costelytra zealandica) was inhibited by phthaleins, sulphonphthaleins and some dicarboxylic acids. 2. These compounds had no detectable action on the enzyme from sheep liver. 3. In insect enzyme the inhibition was competitive with respect to glutathione and non-competitive with respect to the aromatic substrate. 4. Michaelis constants and inhibitor constants were measured for sheep-liver or grass-grub enzyme between pH5 and pH10 and evidence was obtained for the presence of two groups with pK9.2 in the glutathione-binding site of the insect enzyme. 5. Only one such group was detected in the sheep-liver enzyme.

Citing Articles

Role of human glutathione transferases in biotransformation of the nitric oxide prodrug JS-K.

Sjodin B, Mannervik B Sci Rep. 2021; 11(1):20765.

PMID: 34675290 PMC: 8531399. DOI: 10.1038/s41598-021-00327-1.

Kinetic studies on a glutathione S-transferase from the larvae of Costelytra zealandica.

Clark A, Dick G, Smith J Biochem J. 1984; 217(1):51-8.

PMID: 6696730 PMC: 1153180. DOI: 10.1042/bj2170051.

Subcellular distribution of hepatic bile acid-conjugating enzymes.

Lim W, Jordan T Biochem J. 1981; 197(3):611-8.

PMID: 6173037 PMC: 1163172. DOI: 10.1042/bj1970611.

Enzymes catalysing conjugations of glutathione with alpha-beta-unsaturated carbonyl compounds.

Boyland E, Chasseaud L Biochem J. 1968; 109(4):651-61.

PMID: 5683512 PMC: 1186951. DOI: 10.1042/bj1090651.

The inhibition of 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) dehydrochlorinase and glutathione S-aryltransferase in grass-grub and housefly preparations.

Balabaskaran S, Smith J Biochem J. 1970; 117(5):989-96.

PMID: 5451919 PMC: 1179060. DOI: 10.1042/bj1170989.

References

Clark A, Hitchcock M, Smith J . Metabolism of gammexane in flies, ticks and locusts. Nature. 1966; 209(5018):103. DOI: 10.1038/209103a0. View

Combes B, STAKELUM G . A liver enzyme that conjugates sulfobromophthalein sodium with glutathione. J Clin Invest. 1961; 40:981-8. PMC: 290815. DOI: 10.1172/JCI104337. View

Andrews P . Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964; 91(2):222-33. PMC: 1202876. DOI: 10.1042/bj0910222. View

Grover P, Sims P . The metabolism of gamma-2,3,4,5,6-pentachlorocyclohex-1-ene and gamma-hexachlorocyclohexane in rats. Biochem J. 1965; 96(2):521-5. PMC: 1207069. DOI: 10.1042/bj0960521. View

Grover P, Sims P . Conjugations with glutathione. Distribution of glutathione S-aryltransferase in vertebrate species. Biochem J. 1964; 90(3):603-6. PMC: 1202741. DOI: 10.1042/bj0900603. View