Ultrahistochemical Study on the Ruthenium Red Surface Staining. I. Processes Which Give Rise to Electron-dense Marker

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1976 May 28

PMID 60315

Citations 10

Authors

P R Blanquet

Affiliations

Soon will be listed here.

Abstract

The cell coat picture effect which is usually obtained with the conventional RR method, that is with the RR/OsO4 coupled reaction, is investigated. In this first paper, each of conceivable events which might take place between RR, OSO4 and cell surface membrane is discussed or studied. Various tests are carried out on ascites Ehrlich carcinoma cells and Zajdela ascites hepatoma cells treated with numerous chemical reagents, as also on a few pure proteins. The set of data supports the concept that the staining pattern is due to the combination in surface membranes of RR with a "colloidal-like" form of OsO2. The latter might occur during the formation of stable cyclic osmic acid diesters between OsO4 and membrane unsaturated lipids. A possibility by which the resulting marker is though also to be in a "colloidal-like" state is put forward. A next report will deal with this problem.

Citing Articles

In focus in HCB.

Taatjes D, Roth J Histochem Cell Biol. 2023; 160(2):79-81.

PMID: 37477835 DOI: 10.1007/s00418-023-02225-1.

The cell coat of the olfactory epithelium proper and vomeronasal neuroepithelium of the rat as revealed by means of the Ruthenium-red reaction.

Mendoza A, Breipohl W Cell Tissue Res. 1983; 230(1):139-46.

PMID: 6189615 DOI: 10.1007/BF00216034.

Oxidation of ruthenium red for use as an intercellular tracer.

Handley D, Chien S Histochemistry. 1981; 71(2):249-58.

PMID: 6165705 DOI: 10.1007/BF00507828.

The cell coat of the developing olfactory epithelium in the chick.

Mendoza A Cell Tissue Res. 1980; 207(2):227-32.

PMID: 6156001 DOI: 10.1007/BF00237807.

Ultrastructural distribution of acidic glycosaminoglycans associated with matrix vesicle-mediated calcification in mouse progenitor predentine.

Kogaya Y, Furuhashi K Calcif Tissue Int. 1985; 37(1):36-41.

PMID: 2581682 DOI: 10.1007/BF02557676.

References

Kelly D . Fine structure of desmosomes. , hemidesmosomes, and an adepidermal globular layer in developing newt epidermis. J Cell Biol. 1966; 28(1):51-72. PMC: 2106892. DOI: 10.1083/jcb.28.1.51. View

HIGHTON T, Myers D, Rayns D . The intercellular spaces of synovial tissue. N Z Med J. 1968; 67(429):315-25. View

RIEMERSMA J . Osmium tetroxide fixation of lipids for electron microscopy. A possible reaction mechanism. Biochim Biophys Acta. 1968; 152(4):718-27. DOI: 10.1016/0005-2760(68)90118-5. View

Korn E . A chromatographic and spectrophotometric study of the products of the reaction of osmium tetroxide with unsaturated lipids. J Cell Biol. 1967; 34(2):627-38. PMC: 2107326. DOI: 10.1083/jcb.34.2.627. View

Schidlovsky G . CONTRAST IN MULTILAYER SYSTEMS AFTER VARIOUS FIXATIONS. Lab Invest. 1965; 14:1213-33. View

Rosenfeld C, Paintrand M, Choquet C, Venuat A . Cyclic variations in the ruthenium red stained coat of cells from a synchronized human lymphoblastoid line. Exp Cell Res. 1973; 79(2):465-8. DOI: 10.1016/0014-4827(73)90472-2. View

Korn E . 3. Modification of oleic acid during fixation of Amoebae by osmium tetroxide. Biochim Biophys Acta. 1966; 116(2):325-35. DOI: 10.1016/0005-2760(66)90015-4. View

LUFT J . Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action. Anat Rec. 1971; 171(3):347-68. DOI: 10.1002/ar.1091710302. View

Martinez-Palomo A, Braislovsky C, Bernhard W . Ultrastructural modifications of the cell surface and intercellular contacts of some transformed cell strains. Cancer Res. 1969; 29(4):925-37. View

10.

KALIFAT S, Delarue J . [Ultrastructural demonstration of polysaccharides, of which some are acid, in the surface film of the pulmonary alveoli]. J Ultrastruct Res. 1970; 32(5):572-89. DOI: 10.1016/s0022-5320(70)80029-6. View

11.

Korn E . II. Synthesis of bis(methyl 9,10-dihydroxystearate)osmate from methyl oleate and osmium tetroxide under conditions used for fixation of biological material. Biochim Biophys Acta. 1966; 116(2):317-24. DOI: 10.1016/0005-2760(66)90014-2. View

12.

HAKE T . STUDIES ON THE REACTIONS OF OSO4 AND KMNO4 WITH AMINO ACIDS, PEPTIDES, AND PROTEINS. Lab Invest. 1965; 14:1208-12. View

13.

Collin R, Griffith W . Letter: Mechanism of tissue component staining by osmium tetroxide. J Histochem Cytochem. 1974; 22(10):992-3. DOI: 10.1177/22.10.992. View

14.

Napolitano L, Lebaron F, Scaletti J . Preservation of myelin lamellar structure in the absence of lipid. A correlated chemical and morphological study. J Cell Biol. 1967; 34(3):817-26. PMC: 2107173. DOI: 10.1083/jcb.34.3.817. View

15.

Fleischer S, Fleischer B, Stoeckenius W . Fine structure of lipid-depleted mitochondria. J Cell Biol. 1967; 32(1):193-208. PMC: 2107081. DOI: 10.1083/jcb.32.1.193. View

16.

VORBRODT A, KOPROWSKI H . Ruthenium red-stained coat of normal and simian virus 40-transformed cells. J Natl Cancer Inst. 1969; 43(6):1241-8. View

17.

WIGGLESWORTH V . The use of osmium in the fixation and staining of tissues. Proc R Soc Lond B Biol Sci. 1957; 147(927):185-99. DOI: 10.1098/rspb.1957.0043. View

18.

Reimann B . [On the value of ruthenium red as a contrast medium for electron microscopy]. Mikroskopie. 1961; 16:224-6. View

19.

Dreher K, SCHULMAN J, Anderson O, Roels O . The stability and structure of mixed lipid monolayers and bilayers. I. Properties of lipid and lipoprotein monolayers on OsO4 solutions and the role of cholesterol, retinol, and tocopherol in stabilizing lecithin monolayers. J Ultrastruct Res. 1967; 19(5):586-99. DOI: 10.1016/s0022-5320(67)80084-4. View

20.

Szubinska B, LUFT J . Ruthenium red and violet. 3. Fine structure of the plasma membrane and extraneous coats in amoebae (A. proteus and Chaos chaos). Anat Rec. 1971; 171(3):417-41. DOI: 10.1002/ar.1091710304. View