Recombination After Protoplast Fusion in the Yeast Candida Tropicalis

Overview

Journal Arch Microbiol

Specialty Microbiology

Date 1977 Nov 18

PMID 596992

Citations 18

Authors

P Fournier

A Provost

C Bourguignon

H Heslot

Affiliations

Soon will be listed here.

Abstract

Candida tropicalis protoplasts obtained by snail enzyme treatment were induced to fuse by the use of polyethylene-glycol. Heterokaryons formed by two auxotrophic strains were selected by complementation on minimal medium. These heterokaryons were unstable and readily dissociated into their nuclear components. Under appropriate conditions, the parental nuclei of an heterokaryon fused. The homokaryon so obtained was unstable and segregated into various types of auxotrophic and prototrophic recombinants.

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