An Examination of the Evidence for Membrane Pores in Frog Skin

Overview

Journal J Physiol

Specialty Physiology

Date 1966 Jul 1

PMID 5965891

Citations 22

Authors

J Dainty

C R House

Affiliations

Soon will be listed here.

Abstract

1. Measurements of the diffusional permeability, P(d), of tritiated water in isolated frog skin bathed in sulphate Ringer have been made under different stirring conditions.2. The mean +/- S.E. values for P(d) were found to be (6.5 +/- 0.3), (7.9 +/- 0.5), (9.7 +/- 0.7) and (11.1 +/- 0.8) x 10(-5) cm sec(-1) at 120, 300, 500 and 1000 rev/min. It is considered that these results indicate the existence of ;unstirred layers' associated with frog skin.3. The hydraulic conductivity, L(p), of the skin in sulphate Ringer was found to be (2.36 +/- 0.07) x 10(-7) cm sec(-1) atm(-1) (+/- S.E. of estimate), and no marked increase in this value for L(p) was found when the stirring rate was increased from 0 to 500 rev/min.4. It is considered that these results show that previous comparisons of the relative magnitudes of L(p)RT/V(w) (where V(w) is the partial molar volume of water) and P(d) for frog skin have been in error because of the presence of ;unstirred layers'.5. The bearing of our results and other evidence on the question of pores in cell membranes has been discussed.

Citing Articles

Rheological Droplet Interface Bilayers (rheo-DIBs): Probing the Unstirred Water Layer Effect on Membrane Permeability via Spinning Disk Induced Shear Stress.

Barlow N, Bolognesi G, Haylock S, Flemming A, Brooks N, Barter L Sci Rep. 2017; 7(1):17551.

PMID: 29242597 PMC: 5730560. DOI: 10.1038/s41598-017-17883-0.

The role of intercellular channels in the transepithelial transfer of water and sodium in the frog urinary bladder.

Jard S, Bourguet J, Favard P, Carasso N J Membr Biol. 2013; 4(1):124-47.

PMID: 24174234 DOI: 10.1007/BF02431966.

The role of water diffusion in the action of vasopressin.

Hays R, Franki N J Membr Biol. 2013; 2(1):263-76.

PMID: 24174151 DOI: 10.1007/BF01869864.

From membrane pores to aquaporins: 50 years measuring water fluxes.

Parisi M, Dorr R, Ozu M, Toriano R J Biol Phys. 2009; 33(5-6):331-43.

PMID: 19669522 PMC: 2565768. DOI: 10.1007/s10867-008-9064-5.

Extracellular Ca2+ and the effect of antidiuretic hormone on the water permeability of the toad urinary bladder: an example of flow-induced alteration of flow.

Hardy M, Dibona D J Membr Biol. 1982; 67(1):27-44.

PMID: 6808140 DOI: 10.1007/BF01868645.

References

Capraro V, Bernini G . Mechanism of action of extracts of the posthypophysis on water transport through the skin of the frog (Rana esculenta). Nature. 1952; 169(4298):454. DOI: 10.1038/169454a0. View

Garby L, Linderholm H . The permeability of frog skin to heavy water and to ions, with special reference to the effect of some diuretics. Acta Physiol Scand. 1953; 28(4):336-46. DOI: 10.1111/j.1748-1716.1953.tb00985.x. View

LOVTRUP S . On the rate of water exchange across the surface of animal cells. J Theor Biol. 1963; 5(3):341-59. DOI: 10.1016/0022-5193(63)90082-1. View

Andersen B, USSING H . Solvent drag on non-electrolytes during osmotic flow through isolated toad skin and its response to antidiuretic hormone. Acta Physiol Scand. 1957; 39(2-3):228-39. DOI: 10.1111/j.1748-1716.1957.tb01425.x. View

HOSHIKO T, LINDLEY B . THE RELATIONSHIP OF USSING'S FLUX-RATIO EQUATION TO THE THERMODYNAMIC DESCRIPTION OF MEMBRANE PERMEABILITY. Biochim Biophys Acta. 1964; 79:301-17. View

Durbin R, Frank H, Solomon A . Water flow through frog gastric mucosa. J Gen Physiol. 1956; 39(4):535-51. PMC: 2147550. DOI: 10.1085/jgp.39.4.535. View

Dainty J, House C . Unstirred layers in frog skin. J Physiol. 1966; 182(1):66-78. PMC: 1357456. DOI: 10.1113/jphysiol.1966.sp007809. View

KOEFOED-JOHNSEN V, USSING H . The contributions of diffusion and flow to the passage of D2O through living membranes; effect of neurohypophyseal hormone on isolated anuran skin. Acta Physiol Scand. 1953; 28(1):60-76. DOI: 10.1111/j.1748-1716.1953.tb00959.x. View

Ginzburg B, KATCHALSKY A . THE FRICTIONAL COEFFICIENTS OF THE FLOWS OF NON-ELECTROLYTES THROUGH ARTIFICIAL MEMBRANES. J Gen Physiol. 1963; 47:403-18. PMC: 2195340. DOI: 10.1085/jgp.47.2.403. View

10.

USSING H . Some aspects of the application of tracers in permeability studies. Adv Enzymol Relat Subj Biochem. 1952; 13:21-65. DOI: 10.1002/9780470122587.ch2. View

11.

House C . THE NATURE OF WATER TRANSPORT ACROSS FROG SKIN. Biophys J. 1964; 4:401-16. PMC: 1367527. DOI: 10.1016/s0006-3495(64)86791-6. View

12.

Kedem O, KATCHALSKY A . Thermodynamic analysis of the permeability of biological membranes to non-electrolytes. Biochim Biophys Acta. 1958; 27(2):229-46. DOI: 10.1016/0006-3002(58)90330-5. View

13.

Whittembury G . Action of antidiuretic hormone on the equivalent pore radius at both surfaces of the epithelium of the isolated toad skin. J Gen Physiol. 1962; 46:117-30. PMC: 2195249. DOI: 10.1085/jgp.46.1.117. View

14.

Macrobbie E, USSING H . Osmotic behaviour of the epithelial cells of frog skin. Acta Physiol Scand. 1961; 53:348-65. DOI: 10.1111/j.1748-1716.1961.tb02293.x. View

15.

Dainty J, Ginzburg B . Irreversible thermodynamics and frictional models of membrane processes, with particular reference to the cell membrane. J Theor Biol. 1963; 5(2):256-65. DOI: 10.1016/0022-5193(63)90063-8. View