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Beta-glucosidase of Trichoderma: Its Biosynthesis and Role in Saccharification of Cellulose

Overview
Journal Appl Environ Microbiol
Specialties Environmental Health
Microbiology
Date 1976 May 1
PMID 5951
Citations 20
Authors
D Sternberg
Affiliations
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Abstract

The extracellular beta-glucosidase of Trichoderma viride generally is present in low levels when the organism is cultured on cellulose because it is inactivated under the acid conditions which develop in the medium while the other enzymes of the cellulase complex are more stable. With the appropriate pH control, inactivation of beta-glucosidase is prevented and the activity of this enzyme increases during growth. In the saccharification of crystalline cellulose, or of cellulose at low concentrations, much of the glucose produced is the result of the cleavage of cellobiose by beta-glucosidase. However when high concentrations (10%) of pretreated cellulose are saccharified, significant quantities of glucose are produced by action of enzymes other than beta-glucosidase.

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