The Effect of Hexokinase and Tricarboxylic Acid-cycle Intermediates on Fatty Acid Oxidation and Formation of Ketone Bodies by Rat-liver Mitochondria

Overview

Journal Biochem J

Specialty Biochemistry

Date 1966 Feb 1

PMID 5944642

Citations 1

Authors

F J Hird

R H Symons

M J Weidemann

Affiliations

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Abstract

1. The oxidation of butyrate, hexanoate and octanoate by rat-liver mitochondria suspended in a tris-potassium chloride medium in the presence of malate and serum albumin has been investigated. 2. The oxidation of butyrate to acetoacetate was markedly decreased by the addition of a system competitive for ATP (hexokinase-glucose). 3. Serum albumin or tricarboxylic acid-cycle intermediates prevented the inhibition by hexokinase and in their presence a greater proportion of the oxygen consumption was contributed by the tricarboxylic acid cycle. The results suggest that the energy supply for fatty acid activation is either compartmentalized in a spatial or kinetic sense or there exists a special activating mechanism not involving ATP. 4. Malate and other tricarboxylic acid-cycle intermediates caused substantial reduction (to beta-hydroxybutyrate) of the acetoacetate formed during the oxidation of butyrate, hexanoate and octanoate.

Citing Articles

Oxidative phosphorylation accompanying oxidation of short-chain fatty acids by rat-liver mitochondria.

Hird F, Weidemann M Biochem J. 1966; 98(2):378-88.

PMID: 4223170 PMC: 1264855. DOI: 10.1042/bj0980378.

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