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Modified Pagano Levin Medium to Isolate Candida Species

Overview
Journal Appl Microbiol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1966 Jul 1
PMID 5927025
Citations 5
Authors
M A Stedham
D C KELLEY
E H COLES
Affiliations
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Abstract

The use of Pagano Levin medium for primary isolation of Candida species from highly contaminated specimens proved unsatisfactory owing to overgrowth by bacteria and fungi. Addition of cycloheximide and chloramphenicol to the medium greatly improved results by inhibiting overgrowth. The additional inhibitors also altered rate of growth and degree of pigmentation reported for some of the Candida species.

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Isolation and detection of multiple yeasts from a single clinical sample by use of Pagano-Levin agar medium.

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Comparison of Sabouraud dextrose and Pagano-Levin agar media for detection and isolation of yeasts from oral samples.

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References
1.
GEORG L, Ajello L, PAPAGEORGE C . Use of cycloheximide in the selective isolation of fungi pathogenic to man. J Lab Clin Med. 1954; 44(3):422-8. View
2.
PAGANO J, LEVIN J, TREJO W . Diagnostic medium for differentiation of species of Candida. Antibiot Annu. 1957; 5:137-43. View
3.
McLean I, Schwab J, HILLEGAS A, SCHLINGMAN A . SUSCEPTIBILITY OF MICRO-ORGANISMS TO CHLORAMPHENICOL (CHLOROMYCETIN). J Clin Invest. 1949; 28(5 Pt 1):953-63. PMC: 438931. DOI: 10.1172/JCI102185. View