The Action of Ouabain on the Smooth Muscle Cells of the Guinea-pig's Taenia Coli

Overview

Journal J Physiol

Specialty Physiology

Date 1966 May 1

PMID 5921534

Citations 25

Authors

R Casteels

Affiliations

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Abstract

1. The action of 10(-6) g/ml. of ouabain on the membrane potential, membrane activity, tension development and the ion content of the smooth muscle of the guinea-pig's taenia coli has been investigated.2. This ouabain concentration produced a depolarization of the membrane, accompanied by an initial increase of the spike frequency and later a depolarization block. Removal of the ouabain produced a sudden and pronounced hyperpolarization leading to a block of spike discharge.3. Reduction of the external sodium concentration, or an increase of the external calcium concentration, or exposure to 10(-7) g/ml. adrenaline repolarized the cell membrane in the presence of ouabain.4. Ouabain decreased the tension development of the taenia coli.5. Ouabain decreased the intracellular potassium and chloride and increased the intracellular sodium concentration. However, the corresponding changes of the equilibrium potentials were not sufficient to explain the changes of the membrane potential.6. A possible explanation for the action of ouabain is presented suggesting that the drug may decrease the calcium binding properties of the cell membrane.

Citing Articles

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The action of isoprenaline on the smooth muscle of the guinea-pig taenia coli.

BULBRING E, Den Hertog A J Physiol. 1980; 304:277-96.

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Effects of sodium gradient manipulation upon cellular calcium, 45Ca fluxes and cellular sodium in the guinea-pig taenia coli.

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A possible role of linked Na and Cl movement in active Cl uptake in smooth muscle.

Widdicombe J, Brading A Pflugers Arch. 1980; 386(1):35-7.

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Effect of sodium concentration and of atropine on the contractile response of the guinea-pig ileum to potassium ions.

Shimuta S, Nouailhetas V, Valero V, Paiva A, Paiva T Pflugers Arch. 1982; 394(2):186-90.

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