Physical Properties of Tamm-Horsfall Glycoprotein and Its Glycopolypeptide

The molecular weight of the constituent glycopolypeptide chain of T-H glycoprotein was determined by sedimentation equilibrium under two entirely different sets of denaturing conditions. For both sets of denaturing conditions, the average molecular weight estimated for T-H glycopolypeptide was 74,000. The gel chromatographic behavior in 6M guanidium chloride of T-H glycopolypeptide with disulfide bonds intact as compared with its gel chromatographic behavior with disulfide bonds broken indicated that the glycopolypeptide is highly constrained by intrachain disulfide bonds.