The Effect of Oophorectomy on the Ribonucleic Acid Metabolism of a Transplantable Uterine Tumour

Overview

Journal Biochem J

Specialty Biochemistry

Date 1965 Dec 1

PMID 5894043

Citations 1

Authors

W I MAINWARING

D C Williams

Affiliations

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Abstract

1. The incorporation of [(14)C]phenylalanine and of [(3)H]uridine into the Guerin T8 uterine carcinoma has been examined. 2. The addition in vitro of polyuridylic acid caused an increase in the incorporation of labelled phenylalanine into tumour microsomes obtained from both oophorectomized and intact animals. 3. Oophorectomy of tumour-bearing animals decreased the specific activity due to the uptake in vivo of [(3)H]uridine into both the soluble and insoluble RNA of the tumour. 4. Actinomycin D caused a decrease in specific activity of RNA in the tumour in vivo, and puromycin inhibited uptake in vitro into tumour microsomes.

Citing Articles

The effect of castration on the ribonucleic acid metabolism of an experimental prostatic tumour.

MAINWARING W, Williams D Biochem J. 1966; 98(3):836-40.

PMID: 5950019 PMC: 1264926. DOI: 10.1042/bj0980836.

References

GREENMAN D, Kenney F . EFFECTS OF ALTERATIONS IN HORMONAL STATUS ON RIBOSOMES OF RAT UTERUS. Arch Biochem Biophys. 1964; 107:1-6. DOI: 10.1016/0003-9861(64)90261-9. View

Warner J, Rich A, Hall C . Electron Microscope Studies of Ribosomal Clusters Synthesizing Hemoglobin. Science. 1962; 138(3548):1399-403. DOI: 10.1126/science.138.3548.1399. View

Yarmolinsky M, Haba G . INHIBITION BY PUROMYCIN OF AMINO ACID INCORPORATION INTO PROTEIN. Proc Natl Acad Sci U S A. 1959; 45(12):1721-9. PMC: 222790. DOI: 10.1073/pnas.45.12.1721. View

Penman S, Scherrer K, Becker Y, Darnell J . POLYRIBOSOMES IN NORMAL AND POLIOVIRUS-INFECTED HELA CELLS AND THEIR RELATIONSHIP TO MESSENGER-RNA. Proc Natl Acad Sci U S A. 1963; 49(5):654-62. PMC: 299946. DOI: 10.1073/pnas.49.5.654. View

Kidson C, KIRBY K, Ralph R . ISOLATION CHARACTERISTICS OF RAPIDLY LABELLED RNA FROM NORMAL RAT LIVER. J Mol Biol. 1963; 7:312-5. DOI: 10.1016/s0022-2836(63)80010-8. View

Liao S, WILLIAMS-ASHMAN H . An effect of testosterone on amino acid incorporation by prostatic ribonucleoprotein particles. Proc Natl Acad Sci U S A. 1962; 48:1956-64. PMC: 221104. DOI: 10.1073/pnas.48.11.1956. View

Reich E, Franklin R, Shatkin A, TATUMEL . Action of actinomycin D on animal cells and viruses. Proc Natl Acad Sci U S A. 1962; 48:1238-45. PMC: 220938. DOI: 10.1073/pnas.48.7.1238. View

RENDI R, Hultin T . Preparation and amino acid incorporating ability of ribonucleoprotein-particles from different tissues of the rat. Exp Cell Res. 1960; 19:253-66. DOI: 10.1016/0014-4827(60)90006-9. View

WIDNELL C, Tata J . A procedure for the isolation of enzymically active rat-liver nuclei. Biochem J. 1964; 92(2):313-7. PMC: 1205996. DOI: 10.1042/bj0920313. View

10.

Talwar G, Segal S, Evans A, Davidson O . THE BINDING OF ESTRADIOL IN THE UTERUS: A MECHANISM FOR DEPRESSION OF RNA SYNTHESIS. Proc Natl Acad Sci U S A. 1964; 52:1059-66. PMC: 300395. DOI: 10.1073/pnas.52.4.1059. View

11.

Moore R, Hamilton T . ESTROGEN-INDUCED FORMATION OF UTERINE RIBOSOMES. Proc Natl Acad Sci U S A. 1964; 52:439-46. PMC: 300297. DOI: 10.1073/pnas.52.2.439. View

12.

DOUNCE A . THE ISOLATION OF NUCLEI FROM TUMOR CELLS. Exp Cell Res. 1963; 24:SUPPL9:126-43. DOI: 10.1016/0014-4827(63)90253-2. View

13.

Warner J, Knopf P, Rich A . A multiple ribosomal structure in protein synthesis. Proc Natl Acad Sci U S A. 1963; 49:122-9. PMC: 300639. DOI: 10.1073/pnas.49.1.122. View

14.

Kidson C, KIRBY K . RECOGNITION OF ALTERED PATTERNS OF MESSENGER RNA SYNTHESIS IN A MOUSE HEPATOMA. Cancer Res. 1964; 24:1604-9. View

15.

CAMPBELL P, Cooper C, Hicks M . Studies on the role of the morphological constituents of the microsome fraction from rat liver in protein synthesis. Biochem J. 1964; 92(2):225-34. PMC: 1205986. DOI: 10.1042/bj0920225. View

16.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

17.

Sorm F, KEILOVA H . The anti-tumour activity of 6-azauracil riboside. Experientia. 1958; 14(6):215. DOI: 10.1007/BF02159094. View

18.

Gorski J . EARLY ESTROGEN EFFECTS ON THE ACTIVITY OF UTERINE RIBONUCLEIC ACID POLYMERASE. J Biol Chem. 1964; 239:889-92. View

19.

Munro A, Jackson R, Korner A . Studies on the nature of polysomes. Biochem J. 1964; 92(2):289-99. PMC: 1205993. DOI: 10.1042/bj0920289. View