A Rat Liver System That Catalyses a Pyridoxal Phosphate-independent Alpha Beta-eliminto

Overview

Journal Biochem J

Specialty Biochemistry

Date 1969 Sep 1

PMID 5810088

Citations 10

Authors

Tudballn

J H Thmas

J A Fowler

Affiliations

Soon will be listed here.

Abstract

1. Attempts were made to demonstrate the presence of pyridoxal phosphate in the rat liver system catalysing the alphabeta-elimination of l-serine O-sulphate. 2. Methods designed to resolve protein-bound cofactor, spectroscopic examination of a purified enzyme system and attempted reactivation of apo-(alanine aminotransferase) failed to demonstrate the presence of pyridoxal phosphate. 3. The activity of the alphabeta-eliminating system remained constant in vitamin B(6)-deficient animals even though the activities of other pyridoxal phosphate-dependent systems fell markedly. 4. The metabolism of l-serine O[(35)S]-sulphate in vivo appears to be normal in vitamin B(6)-deficient animals. 5. No incorporation of tritium into the alphabeta-eliminating system occurred after administration of tritiated pyridoxine to experimental animals.

Citing Articles

Inhibition of growth of Salmonella typhimurium and of threonine deaminase and transaminase B by beta-chloroalanine.

ARFIN S, Koziell D J Bacteriol. 1971; 105(2):519-22.

PMID: 5541528 PMC: 248412. DOI: 10.1128/jb.105.2.519-522.1971.

The enzymic and non-enzymic cleavage of N-terminal L-serine O-sulphate.

Tudball N, Thomas J Biochem J. 1970; 120(4):895-7.

PMID: 5500355 PMC: 1179685. DOI: 10.1042/bj1200895.

The alpha beta-elimination of L-serylglycine O-sulphate and related dipeptides.

Thomas J, Tudball N Biochem J. 1970; 119(5):57P.

PMID: 5492833 PMC: 1179536. DOI: 10.1042/bj1190057pa.

Sulphur utilization during growth of pseudomonas fluorescens on potassium D-glucose 6-O-sulphate.

Fitzgerald J, DODGSON K Biochem J. 1971; 121(3):521-8.

PMID: 5119788 PMC: 1176600. DOI: 10.1042/bj1210521.

The purification and properties of the L-serine O-sulphate degrading system of pig liver.

Tudball N, Thomas P, Bailey-Wood R Biochem J. 1971; 121(5):747-52.

PMID: 5113489 PMC: 1176662. DOI: 10.1042/bj1210747.

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