The Metabolism of Aromatic Compounds by Rhodopseudomonas Palustris. A New, Reductive, Method of Aromatic Ring Metabolism

Overview

Journal Biochem J

Specialty Biochemistry

Date 1969 Jul 1

PMID 5807211

Citations 58

Authors

P L Dutton

W C Evans

Affiliations

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Abstract

1. Rhodopseudomonas palustris grows both aerobically and photosynthetically on aromatic acids. p-Hydroxybenzoate and protocatechuate are able to support aerobic growth; these compounds are metabolized by the protocatechuate 4,5-oxygenase pathway. 2. The photoassimilation of benzoate and hydroxybenzoates and the effects of air and darkness on the photoassimilation of benzoate are described. 3. Evidence in conflict with the pathway previously proposed for the photometabolism of benzoate is discussed. 4. The photometabolism of benzoate is accomplished by a novel reductive pathway involving its reduction to cyclohex-1-ene-1-carboxylate, followed by hydration to 2-hydroxycyclohexanecarboxylate and after dehydrogenation to 2-oxocyclohexanecarboxylate further hydration results in ring-fission and the production of pimelate. 5. Attempts were made to prepare cell-free extracts capable of dissimilating benzoate.

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References

Hutner S . Anaerobic and aerobic growth of purple bacteria (Athiorhodaceae) in chemically defined media. J Gen Microbiol. 1950; 4(3):286-93. DOI: 10.1099/00221287-4-3-286. View

Babior B, Bloch K . Aromatization of cyclohexanecarboxylic acid. J Biol Chem. 1966; 241(16):3643-51. View

Rothera A . Note on the sodium nitro-prusside reaction for acetone. J Physiol. 1908; 37(5-6):491-4. PMC: 1533603. DOI: 10.1113/jphysiol.1908.sp001285. View

Dutton P, Evans W . Dissimilation of aromatic substrates by Rhodopseudomonas palustris. Biochem J. 1967; 104(2):30P-31P. PMC: 1270679. View

Hegeman G . The metabolism of p-hydroxybenzoate by Rhodopseudomonas palustris and its regulation. Arch Mikrobiol. 1967; 59(1):143-8. DOI: 10.1007/BF00406325. View

Harary I . Bacterial fermentation of nicotinic acid. I. End products. J Biol Chem. 1957; 227(2):815-22. View

LASCELLES J . The synthesis of porphyrins and bacteriochlorophyll by cell suspensions of Rhodopseudomonas spheroides. Biochem J. 1956; 62(1):78-93. PMC: 1274515. DOI: 10.1042/bj0620078. View

Harary I . Bacterial degradation of nicotinic acid. Nature. 1956; 177(4503):328-9. DOI: 10.1038/177328a0. View

Hughes D . A press for disrupting bacteria and other micro-organisms. Br J Exp Pathol. 1951; 32(2):97-109. PMC: 2073399. View

10.

DAGLEY S, Chapman P, Gibson D, Wood J . DEGRADATION OF THE BENZENE NUCLEUS BY BACTERIA. Nature. 1964; 202:775-8. DOI: 10.1038/202775a0. View

11.

DAGLEY S, Evans W, Ribbons D . New pathways in the oxidative metabolism of aromatic compounds by microorganisms. Nature. 1960; 188:560-6. DOI: 10.1038/188560a0. View

12.

CLARK F, FINA L . The anaerobic decomposition of benzoic acid during methane fermentation. Arch Biochem Biophys. 1952; 36(1):26-32. DOI: 10.1016/0003-9861(52)90374-3. View

13.

Knight M . The photometabolism of propionate by Rhodospirillum rubrum. Biochem J. 1962; 84:170-85. PMC: 1243638. DOI: 10.1042/bj0840170. View

14.

Chakravarti R . Studies in the terpenes; a synthesis of dl-2: 2: 3-trimethylcyclohexane-l-carboxylic acid. J Chem Soc. 1947; 1:1565-8. View

15.

Tsai L, Pastan I, Stadtman E . Nicotinic acid metabolism. II. The isolation and characterization of intermediates in the fermentation of nicotinic acid. J Biol Chem. 1966; 241(8):1807-13. View

16.

Evans W . THE MICROBIOLOGICAL DEGRADATION OF AROMATIC COMPOUNDS. J Gen Microbiol. 1963; 32:177-84. DOI: 10.1099/00221287-32-2-177. View

17.

Harary I . Bacterial fermantation of nicotinic acid. II. Anaerobic reversible hydroxylation of nicotinic acid to 6-hydroxynicotinic acid. J Biol Chem. 1957; 227(2):823-31. View

18.

Dutton P, Evans W . The photometabolism of benzoic acid by Rhodopseudomonas palustris: a new pathway of aromatic ring metabolism. Biochem J. 1968; 109(2):5P-6P. PMC: 1186804. DOI: 10.1042/bj1090005p. View

19.

VAN NIEL C . THE CULTURE, GENERAL PHYSIOLOGY, MORPHOLOGY, AND CLASSIFICATION OF THE NON-SULFUR PURPLE AND BROWN BACTERIA. Bacteriol Rev. 1944; 8(1):1-118. PMC: 440875. DOI: 10.1128/br.8.1.1-118.1944. View