RNA Synthesis in Chinese Hamster Cells. II. Increase in Rate of RNA Synthesis During G1

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1969 Jul 1

PMID 5795885

Citations 11

Authors

M D Enger

R A Tobey

Affiliations

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Abstract

Cultures of mitotic Chinese hamster cells, prepared by mechanical selection, were pulse-labeled with methionine-methyl-(14)C or with uridine-(3)H at different stages in the life cycle. The rate of (14)C incorporation into 18S RNA was measured, as was the rate of uridine-(3)H incorporation into total RNA for both monolayer and suspension cultures. The rate of incorporation increased continuously throughout interphase in a fashion inconsistent with a gene-dosage effect upon RNA synthesis.

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