Fatty Acid Desaturase Mutants of Saccharomyces Cerevisiae

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1969 May 1

PMID 5784201

Citations 20

Authors

A D Keith

M R Resnick

A B Haley

Affiliations

Soon will be listed here.

Abstract

Genetic and biochemical analyses were conducted on fatty acid mutants of yeast deficient for Delta(9)-desaturase activity in the production of palmitoleate and oleate. Two genetic loci were observed and two others are inferred; three of these were represented by respiratory-deficient (petite) strains. All strains were incapable of converting palmitate to palmitoleate and stearate to oleate whether the direct precursor or acetate was followed. All strains were capable of acylating both de novoproduced fatty acids and oleate taken up from the medium into phospholipids and neutral lipids. Two revertants were analyzed which differed in their ability to produce palmitoleate and oleate.

Citing Articles

Temperature-sensitive Saccharomyces cerevisiae mutant defective in lipid biosynthesis.

Letts V, Dawes I J Bacteriol. 1983; 156(1):212-21.

PMID: 6352678 PMC: 215072. DOI: 10.1128/jb.156.1.212-221.1983.

Biogenesis of mitochondria: defective yeast H+-ATPase assembled in the absence of mitochondrial protein synthesis is membrane associated.

Orian J, Hadikusumo R, Marzuki S, Linnane A J Bioenerg Biomembr. 1984; 16(5-6):561-81.

PMID: 6242247 DOI: 10.1007/BF00743246.

Double-bond requirement in a fatty acid desaturase mutant of Saccharomyces cerevisiae.

Wisnieski B, Keith A, Resnick M J Bacteriol. 1970; 101(1):160-5.

PMID: 5411750 PMC: 250465. DOI: 10.1128/jb.101.1.160-165.1970.

Isolation and characterization of a phosphatidylethanolamine-deficient mutnt of Bacillus subtilis.

Beebe J J Bacteriol. 1971; 107(3):704-11.

PMID: 4999411 PMC: 246991. DOI: 10.1128/jb.107.3.704-711.1971.

Biogenesis of mitochondria. 19. The effects of unsaturated fatty acid depletion on the lipid composition and energy metabolism of a fatty acid desaturase mutant of Saccharomyces cerevisiae.

Proudlock J, Haslam J, Linnane A J Bioenerg. 1971; 2(5):327-49.

PMID: 4950513 DOI: 10.1007/BF01963829.

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