Effect of Immunosuppressive Agents on Normal Phage-neutralizing Antibody in the Mouse

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1968 Dec 1

PMID 5724964

Authors

R D Karp

S G BRADLEY

Affiliations

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Abstract

The actions of three immunosuppressive drugs on normal antibody synthesis in the adult mouse were determined. Inbred mice were given daily intraperitoneal injections of actinomycin D, uracil mustard, or cyclophosphamide for extended periods. The sera of treated and untreated mice were assayed for phage-neutralizing activity to monitor the effect of each drug on the amount of circulating normal antibody. Except for an initial decrease in titer of normal anti-phage MSP2 activity, actinomycin D had no significant effect on normal antibody activity. Uracil mustard caused alternating elevation and depression of normal antibody titers. Cyclophosphamide caused a prolonged depression of normal antibody. The response patterns to the three immunosuppressive agents were the same for both induced and normal antibody.

References

BUSKIRK H, Crim J, Petering H, Merritt K, Johnson A . Effect of uracil mustard and several antitumor drugs on the primary antibody response in rats and mice. J Natl Cancer Inst. 1965; 34(6):747-58. View

Claman H, Bronsky E . Inhibition of antibody production and acquired immunologic tolerance by actinomycin. J Immunol. 1965; 95(4):718-21. View

MUSCHEL L, Jackson J, SCHMOKER K . Effect of actinomycin D on immune antibody, normal antibody, and complement. J Bacteriol. 1966; 91(1):270-2. PMC: 315944. DOI: 10.1128/jb.91.1.270-272.1966. View

FRISCH A, Davies G . Inhibition of hemagglutin synthesis by cytoxan: specificity and drug-induced "tolerance". J Lab Clin Med. 1966; 68(1):103-12. View

Aisenberg A . Studies on cyclophosphamide-induced tolerance to sheep erythrocytes. J Exp Med. 1967; 125(5):833-45. PMC: 2138214. DOI: 10.1084/jem.125.5.833. View

MILLER 3rd J, Cole L . Resistance of long-lived lymphocytes and plasma cells in rat lymph nodes to treatment with prednisone, cyclophosphamide, 6-mercaptopurine, and actinomycin D. J Exp Med. 1967; 126(1):109-25. PMC: 2138307. DOI: 10.1084/jem.126.1.109. View

Cirkovic D, SIMIC M . Effects of actinomycin D on the primary hemolysin response in rats. J Immunol. 1967; 99(2):327-32. View

Aisenberg A, Wilkes B . Immunological tolerance induced by cyclophosphamide assayed by plaque spleen cell method. Nature. 1967; 213(5075):498-9. DOI: 10.1038/213498a0. View

WUST C, GALL C, NOVELLI D . ACTINOMYCIN D: EFFECT ON THE IMMUNE RESPONSE. Science. 1964; 143(3610):1041-3. DOI: 10.1126/science.143.3610.1041. View

10.

BROOKE M . NATURAL HAEMAGGLUTININS IN MICE: THEIR OCCURRENCE AND PROPERTIES. Immunology. 1965; 8:375-83. PMC: 1423499. View

11.

Michael J, WHITBY J, Landy M . Studies on natural antibodies to gram-negative bacteria. J Exp Med. 1962; 115:131-46. PMC: 2137482. DOI: 10.1084/jem.115.1.131. View

12.

BRADLEY S, Watson D . PRODUCTION OF NEUTRALIZING ANTIBODY BY MICE INJECTED WITH ACTINOPHAGE. J Immunol. 1963; 90:782-7. View