DNA Polymerase Alpha and Beta in the California Urchin

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1978 Oct 1

PMID 569291

Citations 4

Authors

F M Racine

P W MORRIS

Affiliations

Soon will be listed here.

Abstract

DNA polymerase alpha and beta were identified in the urchin, Strongylocentrotus purpuratus. The DNA polymerase beta sedimented at 3.4 S, constituted 5% of total DNA polymerase activity, and was resistant to N-ethylmaleimide and high ionic strength. The polymerase alpha sedimented at 6--8 S, was inhibited by N-ethylmalemide or 0.1 M (NH4)2SO4, and was dependent upon glycerol for preservation of activity. Both the polymerases alpha and beta were nuclear associated in embryos. The DNA polymerase alpha was markedly heterogeneous on DEAE-Sephadex ion exchange and showed three modal polymerase species. These polymerase alpha species were indistinguishable by template activity assays but the DNA polymerase associated ribonucleotidyl transferase (Biochemistry 75 : 3106-3113, 1976) was found predominantly with only one of the DNA polymerase alpha species.

Citing Articles

DNA polymerases in prokaryotes and eukaryotes: mode of action and biological implications.

Hubscher U Experientia. 1983; 39(1):1-25.

PMID: 6297955 DOI: 10.1007/BF01960616.

Multiple polymorphic alpha- and beta-tubulin mRNAs are present in sea urchin eggs.

Alexandraki D, Ruderman J Proc Natl Acad Sci U S A. 1985; 82(1):134-8.

PMID: 3855535 PMC: 396986. DOI: 10.1073/pnas.82.1.134.

The small subunit of ribonucleotide reductase is encoded by one of the most abundant translationally regulated maternal RNAs in clam and sea urchin eggs.

Standart N, Bray S, George E, Hunt T, Ruderman J J Cell Biol. 1985; 100(6):1968-76.

PMID: 2987274 PMC: 2113603. DOI: 10.1083/jcb.100.6.1968.

The mode of inhibitory action by pyridoxal 5-phosphate on DNA polymerase-alpha and -beta.

Oguro M, Nagano H, Mano Y Nucleic Acids Res. 1979; 7(3):727-34.

PMID: 503844 PMC: 328051. DOI: 10.1093/nar/7.3.727.

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