Polyribosomes of Hamster Cells: Transit Time Measurements

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1968 Feb 1

PMID 5689103

Citations 6

Authors

C P Stanners

Affiliations

Soon will be listed here.

Abstract

The amount of radioactivity incorporated into completed polypeptides and into nascent polypeptides bound to polyribosomes was measured for rapidly dividing, cultured hamster embryo cells incubated in the presence of labelled amino acids. Using a mathematical analysis which took into account variations in the specific activity of the intracellular amino acid pool, these measurements yielded a value for the length of time required for the synthesis of a primary polypeptide of average length, the "transit time." Subject to a number of reasonable assumptions in the mathematical analysis which were, however, only approximately verified, the transit time was found to be 23 +/- 2 sec in four independent experiments. Regardless of the absolute accuracy of this measurement, the method is considered a useful measure of translation independent of transcription.

Citing Articles

Glutamatergic Transmission: A Matter of Three.

Martinez-Lozada Z, Ortega A Neural Plast. 2015; 2015:787396.

PMID: 26345375 PMC: 4539489. DOI: 10.1155/2015/787396.

Cytoplasmic synthesis of nuclear proteins. Kinetics of accumulation of radioactive proteins in various cell fractions after brief pulses.

Wu R, Warner J J Cell Biol. 1971; 51(3):643-52.

PMID: 5128352 PMC: 2108027. DOI: 10.1083/jcb.51.3.643.

Inhibitors of ribonucleic acid synthesis in Saccharomyces cerevisiae: decay rate of messenger ribonucleic acid.

Tonnesen T, Friesen J J Bacteriol. 1973; 115(3):889-96.

PMID: 4580571 PMC: 246333. DOI: 10.1128/jb.115.3.889-896.1973.

A mammalian cell mutant with a temperature-sensitive leucyl-transfer RNA synthetase.

Thompson L, HARKINS J, Stanners C Proc Natl Acad Sci U S A. 1973; 70(11):3094-8.

PMID: 4361675 PMC: 427177. DOI: 10.1073/pnas.70.11.3094.

Uptake of amino acids and thymidine during the first cell cycle of synchronized hamster cells.

Bhargava P, Allin E, Montagnier L J Membr Biol. 1975; 26(1):1-17.

PMID: 1221129 DOI: 10.1007/BF01868862.

References

Taylor J, Stanners C . A note on the incorporation of amino acids by hamster embryo cells in monolayer culture. Biochim Biophys Acta. 1967; 138(3):627-30. DOI: 10.1016/0005-2787(67)90566-7. View

Salb J, Marcus P . Translational inhibition in mitotic HeLa cells. Proc Natl Acad Sci U S A. 1965; 54(5):1353-8. PMC: 219902. DOI: 10.1073/pnas.54.5.1353. View

Nathans D . PUROMYCIN INHIBITION OF PROTEIN SYNTHESIS: INCORPORATION OF PUROMYCIN INTO PEPTIDE CHAINS. Proc Natl Acad Sci U S A. 1964; 51:585-92. PMC: 300121. DOI: 10.1073/pnas.51.4.585. View

Penman S, Scherrer K, Becker Y, Darnell J . POLYRIBOSOMES IN NORMAL AND POLIOVIRUS-INFECTED HELA CELLS AND THEIR RELATIONSHIP TO MESSENGER-RNA. Proc Natl Acad Sci U S A. 1963; 49(5):654-62. PMC: 299946. DOI: 10.1073/pnas.49.5.654. View

KANO-SUEOKA T, Sueoka N . Modification of leucyl-sRNA after bacteriophage infection. J Mol Biol. 1966; 20(1):183-209. DOI: 10.1016/0022-2836(66)90124-0. View

Goldstein A, Goldstein D, LOWNEY L . PROTEIN SYNTHESIS OF 0 DEGREES C IN ESCHERICHIA COLI. J Mol Biol. 1964; 9:213-35. DOI: 10.1016/s0022-2836(64)80102-9. View

Bautz E, Kasai T, Reilly E, BAUTZ F . Gene-specific mRNA. II. Regulation of mRNA synthesis in E. coli after infection with bacteriophage T4. Proc Natl Acad Sci U S A. 1966; 55(5):1081-8. PMC: 224282. DOI: 10.1073/pnas.55.5.1081. View

Colombo B, Felicetti L, Baglioni C . INHIBITION OF PROTEIN SYNTHESIS BY CYCLOHEXIMIDE IN RABBIT RETICULOCYTES. Biochem Biophys Res Commun. 1965; 18:389-95. DOI: 10.1016/0006-291x(65)90719-9. View

Conconi F, Bank A, Marks P . Polyribosomes and control of protein synthesis: effects of sodium fluoride and temperature of reticulocytes. J Mol Biol. 1966; 19(2):525-40. DOI: 10.1016/s0022-2836(66)80020-7. View

10.

Gross P, MALKIN L, Moyer W . TEMPLATES FOR THE FIRST PROTEINS OF EMBRYONIC DEVELOPMENT. Proc Natl Acad Sci U S A. 1964; 51:407-14. PMC: 300086. DOI: 10.1073/pnas.51.3.407. View

11.

Reader R, Stanners C . On the significance of ribosome dimers in extracts of animal cells. J Mol Biol. 1967; 28(2):211-23. DOI: 10.1016/s0022-2836(67)80004-4. View

12.

Warner J, Knopf P, Rich A . A multiple ribosomal structure in protein synthesis. Proc Natl Acad Sci U S A. 1963; 49:122-9. PMC: 300639. DOI: 10.1073/pnas.49.1.122. View

13.

Vogt M, DULBECCO R . VIRUS-CELL INTERACTION WITH A TUMOR-PRODUCING VIRUS. Proc Natl Acad Sci U S A. 1960; 46(3):365-70. PMC: 222842. DOI: 10.1073/pnas.46.3.365. View

14.

ABERCROMBIE M, HEAYSMAN J . Observations on the social behaviour of cells in tissue culture. II. Monolayering of fibroblasts. Exp Cell Res. 1954; 6(2):293-306. DOI: 10.1016/0014-4827(54)90176-7. View

15.

DULBECCO R, Vogt M . Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954; 99(2):167-82. PMC: 2180341. DOI: 10.1084/jem.99.2.167. View

16.

McAuslan B . THE INDUCTION AND REPRESSION OF THYMIDINE KINASE IN THE POXVIRUS-INFECTED HELA CELL. Virology. 1963; 21:383-9. DOI: 10.1016/0042-6822(63)90199-5. View

17.

Marks P, Burka E, Conconi F, PERL W, Rifkind R . Polyribosome dissociation and formation in intact reticulocytes with conservation of messenger ribonucleic acid. Proc Natl Acad Sci U S A. 1965; 53(6):1437-43. PMC: 219875. DOI: 10.1073/pnas.53.6.1437. View