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Mass Preparation of Nuclei from the Larval Salivary Glands of Drosophila Hydei

Overview
Journal J Cell Biol
Specialty Cell Biology
Date 1968 Aug 1
PMID 5664209
Citations 18
Authors
J B Boyd
H D BERENDES
H Boyd
Affiliations
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Abstract

A method has been developed for isolating gram quantities of salivary glands from late third instar larvae of Drosophila hydei. The isolated glands have a normal appearance and incorporate RNA and DNA precursors normally. Nuclei can be isolated from these glands in 90% yield with the use of detergents. These nuclei contain morphologically normal giant polytene chromosomes.

Citing Articles

Analysis of nuclear proteins in primary spermatocytes of Drosophila hydei: The correlation of nuclear proteins with the function of the Y chromosomal loops.

Kloetzel P, Knust E, Schwochau M Chromosoma. 1981; 84(1):67-86.

PMID: 7297252 DOI: 10.1007/BF00293364.

Features of in vitro puffing and RNA synthesis in polytene chromosomes of Chironomus.

Sass H Chromosoma. 1980; 78(1):33-78.

PMID: 6155245 DOI: 10.1007/BF00291908.

Structural and functional properties of polytene nuclei isolated from salivary glands of Drosophila hydei.

BERENDES H, Boyd J J Cell Biol. 1969; 41(2):591-9.

PMID: 5783875 PMC: 2107750. DOI: 10.1083/jcb.41.2.591.

Polytene chromosome structure at the submicroscopic level. II. Length distribution of DNA molecules from polytene chromosomes of Drosophila melanogaster and D. hydei.

Derksen J, BERENDES H Chromosoma. 1970; 31(4):468-77.

PMID: 5490312 DOI: 10.1007/BF00285837.

Deoxyribonucleases of the organs of Drosophila hydei at the onset of metamorphosis.

Boyd J, Boyd H Biochem Genet. 1970; 4(4):447-59.

PMID: 5456434 DOI: 10.1007/BF00486595.

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