Cytochemical Studies on RNP Complexes Produced by Puff 2-48BC in Drosophila Hydei: Uranyl Acetate and Phosphotungstic Acid Staining

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 1976 Mar 31

PMID 56256

Citations 3

Authors

J Derksen

E Willart

Affiliations

Soon will be listed here.

Abstract

Differential staining of the core and RNP particles of RNP complexes in puff 2--48 BC in salivary gland chromosomes of Drosophila hydei was achieved with aqueous uranyl acetate (UA) at low pH, with UA in acetone, with phosphotungstic acid (PTA) in organic solvents, and with aqueous PTA at pH 5 And 6. A comparison of the results of UA and PTA staining under various conditions indicate that the proteins in the core region and in the RNP particles connected to it differ with respect to their amino-acid composition (arginine and lysine residues).--The staining mechanism of PTA and UA is discussed.

Citing Articles

A comparative ultrastructural study of 'glue' production and secretion of the salivary glands in different species of theDrosophila melanogaster group.

Thomopoulos G, Kastritsis C Wilehm Roux Arch Dev Biol. 2017; 187(4):329-354.

PMID: 28305005 DOI: 10.1007/BF00848468.

Dynamics of hnRNPs and omega speckles in normal and heat shocked live cell nuclei of Drosophila melanogaster.

Singh A, Lakhotia S Chromosoma. 2015; 124(3):367-83.

PMID: 25663367 DOI: 10.1007/s00412-015-0506-0.

Heat-shock puff 93 D from Drosophila melanogaster: accumulation of a RNP-specific antigen associated with giant particles of possible storage function.

Dangli A, Grond C, Kloetzel P, Bautz E EMBO J. 1983; 2(10):1747-51.

PMID: 6416825 PMC: 555353. DOI: 10.1002/j.1460-2075.1983.tb01652.x.

References

Sarasin A . . FEBS Lett. 1969; 4(4):327-330. DOI: 10.1016/0014-5793(69)80267-x. View

LUFT J . Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961; 9:409-14. PMC: 2224998. DOI: 10.1083/jcb.9.2.409. View

Zobel C, Beer M . Electron stains. I. Chemical studies on the interaction of DNA with uranyl salts. J Biophys Biochem Cytol. 1961; 10:335-46. PMC: 2225082. DOI: 10.1083/jcb.10.3.335. View

Stevens B, Swift H . RNA transport from nucleus to cytoplasm in Chironomus salivary glands. J Cell Biol. 1966; 31(1):55-77. PMC: 2107042. DOI: 10.1083/jcb.31.1.55. View

Martin T, Billings P, Levey A, Ozarslan S, Quinlan T, Swift H . Some properties of RNA:protein complexes from the nucleus of eukaryotic cells. Cold Spring Harb Symp Quant Biol. 1974; 38:921-32. DOI: 10.1101/sqb.1974.038.01.094. View

Silverman L, GLICK D . The reactivity and staining of tissue proteins with phosphotungstic acid. J Cell Biol. 1969; 40(3):761-7. PMC: 2107646. DOI: 10.1083/jcb.40.3.761. View

Beermann W . Nuclear differentiation and functional morphology of chromosomes. Cold Spring Harb Symp Quant Biol. 1956; 21:217-32. DOI: 10.1101/sqb.1956.021.01.018. View

Swift H . CYTOCHEMICAL STUDIES ON NUCLEAR FINE STRUCTURE. Exp Cell Res. 1963; 24:SUPPL9:54-67. DOI: 10.1016/0014-4827(63)90244-1. View

Sheridan W, Barrnett R . Cytochemical studies on chromosome ultrastructure. J Ultrastruct Res. 1969; 27(3):216-29. DOI: 10.1016/s0022-5320(69)80013-4. View

10.

Quintarelli G, Bellocci M, Geremia R . On phosphotungstic acid staining. IV. Selectivity of the staining reaction. J Histochem Cytochem. 1973; 21(2):155-60. DOI: 10.1177/21.2.155. View

11.

Bloom F, Aghajanian G . Fine structural and cytochemical analysis of the staining of synaptic junctions with phosphotungstic acid. J Ultrastruct Res. 1968; 22(5):361-75. DOI: 10.1016/s0022-5320(68)90027-0. View

12.

Hamkalo B, MILLER Jr O . Electronmicroscopy of genetic activity. Annu Rev Biochem. 1973; 42:379-96. DOI: 10.1146/annurev.bi.42.070173.002115. View

13.

Sommerville J . Ribonucleoprotein particles derived from the lampbrush chromosomes of newt oocytes. J Mol Biol. 1973; 78(3):487-503. DOI: 10.1016/0022-2836(73)90470-1. View

14.

Derksen J . The submicroscopic structure of synthetically active units in a puff of drosophila hydei giant chromosomes. Chromosoma. 1975; 50(1):45-52. DOI: 10.1007/BF00284961. View

15.

Bernhard W . Comparative ultrastructural study of perichromatin- and Balbiai rin granules. J Ultrastruct Res. 1971; 36(5):842-60. DOI: 10.1016/s0022-5320(71)90034-7. View

16.

MILLER Jr O, Beatty B . Extrachromosomal nucleolar genes in amphibian oocytes. Genetics. 1969; 61(1):Suppl:133-43. View

17.

Beermann W . [Chromomore constancy and specific modifications of the chromosome structure in development and organ differentiation of Chironomus tentans]. Chromosoma. 1952; 5(2):Unknown. View

18.

BERENDES H, Meyer G . A specific chromosome element, the telomere of Drosophila polytene chromosomes. Chromosoma. 1968; 25(2):184-97. DOI: 10.1007/BF00327177. View

19.

PEASE D . Polysaccharides associated with the exterior surface of epithelial cells: kidney, intestine, brain. J Ultrastruct Res. 1966; 15(5):555-88. DOI: 10.1016/s0022-5320(66)80128-4. View

20.

GABRUSEWYCZ-GARCIA N, Garcia A . Studies on the fine structure of puffs in Sciara coprophila. Chromosoma. 1974; 47(4):385-401. DOI: 10.1007/BF00326361. View