Competitive Interaction of Beta-guanidino Propionic Acid and Gamma-aminobutyric Acid on the Muscle Fibre of the Crayfish

Overview

Journal J Physiol

Specialty Physiology

Date 1971 Jul 1

PMID 5559627

Citations 23

Authors

A Feltz

Affiliations

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Abstract

1. The interaction of beta-guanidino propionic acid and gamma-aminobutyric acid has been investigated on the crayfish muscle by measurement of the membrane conductance.2. The increase in membrane conductance produced by GABA is depressed by beta-guanidino propionic acid. This antagonist acts in a competitive way as shown by the shift of log dose-response curves and by the results of tests for competitive interaction (method of Schild (1947); Lineweaver and Burk's plot).3. Perfusion of GABA was shown to induce a receptor desensitization. As a consequence, the slope of the dose-response curve plotted in Hill's co-ordinates increases when care is taken to minimize this phenomenon. The significance of this slope for establishing the real number of GABA molecules binding with the GABA receptor is discussed.

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