Synergistic Effects of Metabolically Related Amino Acids on the Growth of a Multicellular Plant. II. Studies of 14C-amino Acid Incorporation

Overview

Journal Plant Physiol

Specialty Physiology

Date 1971 Jan 1

PMID 5543785

Citations 11

Authors

V L Dunham

J K Bryan

Affiliations

Soon will be listed here.

Abstract

The synergistic inhibition of the growth of Marchantia polymorpha gemmalings by lysine and threonine and its prevention by methionine has been investigated utilizing (14)C-labeled amino acids. Experiments involving the uptake of (14)C-lysine or (14)C-threonine in the presence or absence of methionine indicated that the synergistic growth effects were not a result of altered amino acid uptake. These data, as well as direct chemical analysis, indicated that growth inhibition was correlated with an inhibition of protein synthesis. Experiments utilizing (14)C-aspartic acid revealed that the presence of lysine and threonine resulted in increased (14)CO(2) production and an accumulation of soluble (14)C-aspartic acid and labeled ninhydrin-positive compounds. These metabolic alterations were prevented when methionine was also included in the growth media. A model depicting a sequence of events which involve the interaction of regulatory mechanisms is suggested to account for the effects of specific amino acids on plant growth.

Citing Articles

Lysine-methionine-threonine interactions in growth and development of Mimulus cardinalis seedlings.

Henke R, Wilson K, McClure J, Treick R Planta. 2014; 116(4):333-45.

PMID: 24458257 DOI: 10.1007/BF00390857.

In-vivo evidence for metabolic control of amino-acid and protein synthesis by exogenous lysine and threonine in Mimulus cardinalis.

Henke R, Wilson K Planta. 2014; 121(2):155-66.

PMID: 24442779 DOI: 10.1007/BF00388754.

Aspartate kinase and the synthesis of aspartate-derived amino acids in wheat.

Bright S, Shewry P, Miflin B Planta. 2014; 139(2):119-25.

PMID: 24414150 DOI: 10.1007/BF00387136.

The effect of aspartate-derived amino acids (lysine, threonine, methionine) on the growth of excised embryos of wheat and barley.

Bright S, WOOD E, Miflin B Planta. 2014; 139(2):113-7.

PMID: 24414149 DOI: 10.1007/BF00387135.

Effects of lysine, threonine and methionine on light-driven protein synthesis in isolated pea (Pisum sativum L.) chloroplasts.

Ronald Mills W, Wilson K Planta. 2014; 142(2):153-60.

PMID: 24408096 DOI: 10.1007/BF00388206.

References

Filner P, Varner J, Wray J . Environmental or developmental changes cause many enzyme activities of higher plants to rise or fall. Science. 1969; 165(3891):358-67. DOI: 10.1126/science.165.3891.358. View

Datta P . Regulation of branched biosynthetic pathways in bacteria. Science. 1969; 165(3893):556-62. DOI: 10.1126/science.165.3893.556. View

BILLEN D, Hewitt R . Influence of starvation for methionine and other amino acids on subsequent bacterial deoxyribonucleic acid replication. J Bacteriol. 1966; 92(3):609-17. PMC: 276298. DOI: 10.1128/jb.92.3.609-617.1966. View

Datta P, Gest H . CONTROL OF ENZYME ACTIVITY BY CONCERTED FEEDBACK INHIBITION. Proc Natl Acad Sci U S A. 1964; 52:1004-9. PMC: 300385. DOI: 10.1073/pnas.52.4.1004. View

Paulus H, Gray E . MULTIVALENT FEEDBACK INHIBITION OF ASPARTOKINASE IN BACILLUS POLYMYXA. J Biol Chem. 1964; 239:PC4008-9. View

Jensen R . Metabolic interlock. Regulatory interactions exerted between biochemical pathways. J Biol Chem. 1969; 244(11):2816-23. View

Lieberman M, Kunishi A . Ethylene production from methionine. Biochem J. 1965; 97(2):449-59. PMC: 1264661. DOI: 10.1042/bj0970449. View

GHOSH H, Preiss J . Adenosine diphosphate glucose pyrophosphorylase. A regulatory enzyme in the biosynthesis of starch in spinach leaf chloroplasts. J Biol Chem. 1966; 241(19):4491-504. View

Scala J, Patrick C, Macbeth G . FDPases of the castor bean endosperm and leaf: properties and partial purification. Arch Biochem Biophys. 1968; 127(1):576-84. DOI: 10.1016/0003-9861(68)90265-8. View

10.

Joy K . Nitrogen metabolism of Lemna minor. I. Growth, nitrogen sources and amino acid inhibition. Plant Physiol. 1969; 44(6):845-8. PMC: 396174. DOI: 10.1104/pp.44.6.845. View

11.

Bryan J . Studies on the catalytic and regulatory properties of homoserine dehydrogenase of Zea mays roots. Biochim Biophys Acta. 1969; 171(2):205-16. DOI: 10.1016/0005-2744(69)90154-5. View

12.

COHEN G, STANIER R, Le Bras G . Regulation of the biosynthesis of amino acids of the aspartate family in Coliform bacteria and Pseudomonads. J Bacteriol. 1969; 99(3):791-801. PMC: 250096. DOI: 10.1128/jb.99.3.791-801.1969. View

13.

Dougall D, Fulton M . Biosynthesis of Protein Amino Acids in Plant Tissue Culture IV Isotope Competition Experiments using Glucose-U-C and Potential Intermediates. Plant Physiol. 1967; 42(7):941-5. PMC: 1086653. DOI: 10.1104/pp.42.7.941. View

14.

BURLANT L, Datta P, Gest H . CONTROL OF ENZYME ACTIVITY IN GROWING BACTERIAL CELLS BY CONCERTED FEEDBACK INHIBITION. Science. 1965; 148(3675):1351-3. DOI: 10.1126/science.148.3675.1351. View

15.

Paulus H, Gray E . Multivalent feedback inhibition of aspartokinase in Bacillus polymyxa. I. Effect of nonpolar L-amino acids. J Biol Chem. 1968; 243(7):1349-55. View

16.

Dunham V, Bryan J . Synergistic effects of metabolically related amino acids on the growth of a multicellular plant. Plant Physiol. 1969; 44(11):1601-8. PMC: 396312. DOI: 10.1104/pp.44.11.1601. View

17.

Oaks A . The synthesis of leucine in maize embryos. Biochim Biophys Acta. 1965; 111(1):79-89. DOI: 10.1016/0304-4165(65)90474-5. View

18.

Oaks A . Asparagine synthesis in Zea mays. Biochim Biophys Acta. 1967; 141(2):436-9. DOI: 10.1016/0304-4165(67)90122-5. View

19.

Radmer R, Bogorad L . (Minus) S-adenosyl-L-methionine-magnesium protoporphyrin methyltransferase, an enzyme in the biosynthetic pathway of chlorophyll in Zea mays. Plant Physiol. 1967; 42(3):463-5. PMC: 1086561. DOI: 10.1104/pp.42.3.463. View

20.

Filner P . Regulation of nitrate reductase in cultured tobacco cells. Biochim Biophys Acta. 1966; 118(2):299-310. DOI: 10.1016/s0926-6593(66)80038-3. View