A Comparison Between Heterogeneous Nuclear RNA and Polysomal Messenger RNA in HeLa Cells by RNA-DNA Hybridization

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1970 Mar 1

PMID 5415231

Citations 11

Authors

R Soeiro

J E Darnell

Affiliations

Soon will be listed here.

Abstract

Heterogeneous nuclear RNA (HnRNA) and mRNA from cytoplasmic polyribosomes of HeLa cells have been compared by RNA-DNA hybridization tests. 1 microg of HeLa cell DNA binds 0.05-0.10 microg of either HnRNA or mRNA. In addition, HeLa DNA that is preexposed to unlabeled HnRNA was found to have a reduced capacity to bind either HnRNA or mRNA. The results are compatible with considerable sequence similarity in the two types of RNA but, as is discussed, firm conclusions are precluded by imperfections of the hybridization reaction as presently employed.

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References

FLAMM W, Walker P, McCallum M . Some properties of the single strands isolated from the DNA of the nuclear satellite of the mouse (Mus musculus). J Mol Biol. 1969; 40(3):423-43. DOI: 10.1016/0022-2836(69)90163-6. View

LATHAM H, Darnell J . Distribution of mRNA in the cytoplasmic polyribosomes of the HeLa cell. J Mol Biol. 1965; 14(1):1-12. DOI: 10.1016/s0022-2836(65)80224-8. View

Attardi G, Parnas H, Hwang M, Attardi B . Giant-size rapidly labeled nuclear ribonucleic acid and cytoplasmic messenger ribonucleic acid in immature duck erythrocytes. J Mol Biol. 1966; 20(1):145-82. DOI: 10.1016/0022-2836(66)90123-9. View

Attardi G, Huang P, KABAT S . Recognition of ribosomal RNA sites in DNA. II. The HeLa cell system. Proc Natl Acad Sci U S A. 1965; 54(1):185-92. PMC: 285818. DOI: 10.1073/pnas.54.1.185. View

Jacob F, MONOD J . Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961; 3:318-56. DOI: 10.1016/s0022-2836(61)80072-7. View

Girard M, LATHAM H, Penman S, Darnell J . ENTRANCE OF NEWLY FORMED MESSENGER RNA AND RIBOSOMES INTO HELA CELL CYTOPLASM. J Mol Biol. 1965; 11:187-201. DOI: 10.1016/s0022-2836(65)80050-x. View

McConkey E, HOPKINS J . Molecular weights of some HeLa ribosomal RNA's. J Mol Biol. 1969; 39(3):545-50. DOI: 10.1016/0022-2836(69)90144-2. View

Scherrer K, LATHAM H, Darnell J . Demonstration of an unstable RNA and of a precursor to ribosomal RNA in HeLa cells. Proc Natl Acad Sci U S A. 1963; 49:240-8. PMC: 299789. DOI: 10.1073/pnas.49.2.240. View

McCarthy B, MCCONAUGHY B . Related base sequences in the DNA of simple and complex organisms. I. DNA-DNA duplex formation and the incidence of partially related base sequences in DNA. Biochem Genet. 1968; 2(1):37-53. DOI: 10.1007/BF01458450. View

10.

Ralph R, Bellamy A . ISOLATION AND PURIFICATION OF UNDEGRADED RIBONUCLEIC ACIDS. Biochim Biophys Acta. 1964; 87:9-16. DOI: 10.1016/0926-6550(64)90041-6. View

11.

Penman S, Scherrer K, Becker Y, Darnell J . POLYRIBOSOMES IN NORMAL AND POLIOVIRUS-INFECTED HELA CELLS AND THEIR RELATIONSHIP TO MESSENGER-RNA. Proc Natl Acad Sci U S A. 1963; 49(5):654-62. PMC: 299946. DOI: 10.1073/pnas.49.5.654. View

12.

Pagoulatos G, Darnell J . A comparison of the heterogeneous nuclear RNA of HeLa cells in different periods of the cell growth cycle. J Cell Biol. 1970; 44(3):476-83. PMC: 2107977. DOI: 10.1083/jcb.44.3.476. View

13.

Britten R, Kohne D . Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968; 161(3841):529-40. DOI: 10.1126/science.161.3841.529. View

14.

Fukada T, KAWADE Y . Characterization of rapidly labeled ribonucleic acid of animal cells in culture. Biochim Biophys Acta. 1965; 103(3):383-98. DOI: 10.1016/0005-2787(65)90132-2. View

15.

Corneo G, Ginelli E, POLLI E . Isolation of the complementary strands of a human satellite DNA. J Mol Biol. 1968; 33(1):331-5. DOI: 10.1016/0022-2836(68)90301-x. View

16.

Eagle H . Amino acid metabolism in mammalian cell cultures. Science. 1959; 130(3373):432-7. DOI: 10.1126/science.130.3373.432. View

17.

Darnell Jr J . Ribonucleic acids from animal cells. Bacteriol Rev. 1968; 32(3):262-90. PMC: 408298. DOI: 10.1128/br.32.3.262-290.1968. View

18.

Soeiro R, Birnboim H, Darnell J . Rapidly labeled HeLa cell nuclear RNA. II. Base composition and cellular localization of a heterogeneous RNA fraction. J Mol Biol. 1966; 19(2):362-72. DOI: 10.1016/s0022-2836(66)80010-4. View

19.

Penman S, Smith I, Holtzman E . Ribosomal RNA synthesis and processing in a particulate site in the HeLa cell nucleus. Science. 1966; 154(3750):786-9. DOI: 10.1126/science.154.3750.786. View

20.

Scherrer K, MARCAUD L . [Remarks on polycistron messenger RNA's in animal cells]. Bull Soc Chim Biol (Paris). 1965; 47(8):1697-713. View