Hydrophobic Proteins of Lamellated Osmiophilic Bodies Isolated from Pig Lung

Overview

Journal Biochem J

Specialty Biochemistry

Date 1979 Dec 1

PMID 540041

Citations 25

Authors

P J PHIZACKERLEY

M H Town

G E NEWMAN

Affiliations

Soon will be listed here.

Abstract

1. In addition to proteins that are insoluble in organic solvents, lamellated bodies isolated from pig lung and surfactant prepared from bronchopulmonary lavage fluid contain another group of proteins that are extracted together with lipid into the organic phase. 2. These hydrophobic proteins constitute about 40% of the total protein of lamellated bodies and about 13% of the total protein of surfactant isolated from lavage fluid, whereas less than 1% of the total protein of pig lung microsomal fraction and mitochondria is extracted by organic solvents. 3. The hydrophobic proteins of lamellated bodies were separated into four fractions and freed from phospholipid by chromatographic procedures. Their apparent molecular weights vary between 11 500 and 16 500, they contain 72--79% of hydrophobic residues and 16--22% of sulphur-containing amino-acids, and leucine is the major N-terminal amino acid in each case.

Citing Articles

The biophysical function of pulmonary surfactant.

Hall S, Zuo Y Biophys J. 2024; 123(12):1519-1530.

PMID: 38664968 PMC: 11213971. DOI: 10.1016/j.bpj.2024.04.021.

Elevated Surfactant Protein Levels and Increased Flow of Cerebrospinal Fluid in Cranial Magnetic Resonance Imaging.

Schob S, Weiss A, Surov A, Dieckow J, Richter C, Pirlich M Mol Neurobiol. 2017; 55(8):6227-6236.

PMID: 29282698 DOI: 10.1007/s12035-017-0835-5.

Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid.

Schob S, Weiss A, Dieckow J, Richter C, Pirlich M, Voigt P Front Aging Neurosci. 2017; 8:324.

PMID: 28101052 PMC: 5209370. DOI: 10.3389/fnagi.2016.00324.

In vitro and in vivo comparison between poractant alfa and the new generation synthetic surfactant CHF5633.

Ricci F, Murgia X, Razzetti R, Pelizzi N, Salomone F Pediatr Res. 2016; 81(2):369-375.

PMID: 27973472 DOI: 10.1038/pr.2016.231.

Staphylococcus aureus and Pseudomonas aeruginosa express and secrete human surfactant proteins.

Brauer L, Schicht M, Worlitzsch D, Bensel T, Sawers R, Paulsen F PLoS One. 2013; 8(1):e53705.

PMID: 23349731 PMC: 3551896. DOI: 10.1371/journal.pone.0053705.

References

Pattle R . SURFACE LINING OF LUNG ALVEOLI. Physiol Rev. 1965; 45:48-79. DOI: 10.1152/physrev.1965.45.1.48. View

BARTLETT G . Phosphorus assay in column chromatography. J Biol Chem. 1959; 234(3):466-8. View

Folch J, Lees M, SLOANE STANLEY G . A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957; 226(1):497-509. View

JEPSON J, Smith I . Multiple dipping procedures in paper chromatography: a specific test for hydroxy-proline. Nature. 1953; 172(4389):1100-1. DOI: 10.1038/1721100b0. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Grathwohl C, NEWMAN G, PHIZACKERLEY P, Town M . Structural studies on lamellated osmiophilic bodies isolated from pig lung. 31P NMR results and water content. Biochim Biophys Acta. 1979; 552(3):509-18. DOI: 10.1016/0005-2736(79)90195-0. View

Morley C, Banhham A, Johnson P, THORBURN G, Jenkin G . Physical and physiological properties of dry lung surfactant. Nature. 1978; 271(5641):162-3. DOI: 10.1038/271162a0. View

Stratton C . The periodicity and architecture of lipid retained and extracted lung surfactant and its origin from multilamellar bodies. Tissue Cell. 1977; 9(2):301-16. DOI: 10.1016/0040-8166(77)90023-4. View

Stratton C . The high resolution ultrastructure of the periodicity and architecture of lipid-retained and extracted lung multilamellar body laminations. Tissue Cell. 1976; 8(4):713-28. DOI: 10.1016/0040-8166(76)90041-0. View

10.

Stratton C . The three-dimensional aspect of the mammalian lung surfactant myelin figure. Tissue Cell. 1977; 9(2):285-300. DOI: 10.1016/0040-8166(77)90022-2. View

11.

Sawada H, Yamabayashi H, Okajima Y . Isolation of apoprotein from bovine lung surfactant material by sodium deoxycholate. Biochim Biophys Acta. 1977; 493(1):196-200. DOI: 10.1016/0005-2795(77)90272-0. View

12.

King R, Martin H, Mitts D, HOLMSTROM F . Metabolism of the apoproteins in pulmonary surfactant. J Appl Physiol Respir Environ Exerc Physiol. 1977; 42(4):483-91. DOI: 10.1152/jappl.1977.42.4.483. View

13.

Sawada H, Kashiwamata S . Sodium dodecyl sulfate-disc gel electrophoresis patterns of bovine lung surfactant. Biochim Biophys Acta. 1977; 490(1):44-50. DOI: 10.1016/0005-2795(77)90104-0. View

14.

Stratton C . The three-dimensional aspect of mammalian lung multilamellar bodies. Tissue Cell. 1976; 8(4):693-712. DOI: 10.1016/0040-8166(76)90040-9. View

15.

SCARPELLI E, Wolfson D, Colacicco G . Protein and lipid-protein fractions of lung washings: immunological characterization. J Appl Physiol. 1973; 34(6):750-3. DOI: 10.1152/jappl.1973.34.6.750. View

16.

Shelley S, LHEUREUX M, BALIS J . Characterization of lung surfactant: factors promoting formation of artifactual lipid-protein complexes. J Lipid Res. 1975; 16(3):224-34. View

17.

Smith D, Smith U, Ryan J . Freeze-fractured lamellar body membranes of the rat lung great alveolar cell. Tissue Cell. 1972; 4(3):457-68. DOI: 10.1016/s0040-8166(72)80022-3. View

18.

King R . The surfactant system of the lung. Fed Proc. 1974; 33(11):2238-47. View

19.

GOERKE J . Lung surfactant. Biochim Biophys Acta. 1974; 344(3-4):241-61. DOI: 10.1016/0304-4157(74)90009-4. View

20.

King R, Klass D, Gikas E, Clements J . Isolation of apoproteins from canine surface active material. Am J Physiol. 1973; 224(4):788-95. DOI: 10.1152/ajplegacy.1973.224.4.788. View