Multiple Active Sites on Human Interferons

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1975 Nov 1

PMID 53841

Citations 13

Authors

K Paucker

B J Dalton

C A OGBURN

E Torma

Affiliations

Soon will be listed here.

Abstract

Human interferons stimulated in peripheral leukocytes and foreskin fibroblasts are active in cultures of human and rabbit cells. The dominant factors in leukocyte and fibroblast interferons responsible for antiviral activity in rabbit cells were shown to be antigenically distinct from each other as well as from rabbit interferon. In addition, leukocyte interferon contained also a minor component with antigenic determinants characteristic of fibroblast specificity, which could be isolated by affinity chromatography on Sepharose-bound antibodies directed against firboblast interferon. Neutralization tests with selected anti-interferon sera suggested that the antiviral activities of leukocyte and fibroblast interferons in human and rabbit cells were associated with single molecules. A model is proposed where molecules of human interferon contain multiple reactive sites each of which is capable of interaction with cells of a different species. The number and distribution of these determinant sites may vary with the source of the human interferon and account for the differential in antiviral protection expressed in homologous and phylogenetically unrelated host cells.

Citing Articles

Biological properties of human interferon fractions obtained by blue sepharose chromatography.

Erickson J, Dalton B, Paucker K Arch Virol. 1980; 63(3-4):253-61.

PMID: 7356397 DOI: 10.1007/BF01315031.

Affinity chromatography of primary human amnion interferon.

Ferreira P, Paucker M, Golgher R, Paucker K Arch Virol. 1981; 68(1):27-33.

PMID: 6166279 DOI: 10.1007/BF01315164.

Lymphocyte blastogenesis and interferon production in adult human leukocyte cultures stimulated with cytomegalovirus antigens.

Starr S, Dalton B, Garrabrant T, Paucker K, Plotkin S Infect Immun. 1980; 30(1):17-22.

PMID: 6160103 PMC: 551270. DOI: 10.1128/iai.30.1.17-22.1980.

Adsorption of interferon to homologous and heterologous cells. Brief report.

DeGre M Arch Virol. 1980; 65(1):71-6.

PMID: 6158931 DOI: 10.1007/BF01340543.

Enhancement of cellular protein synthesis sensitivity to diphtheria toxin by interferon.

Aboud M, Nitzan Y, Salzberg S Infect Immun. 1980; 28(1):11-6.

PMID: 6155343 PMC: 550886. DOI: 10.1128/iai.28.1.11-16.1980.

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