Adherence of Bacterial to Vaginal Epithelial Cells

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1976 Mar 1

PMID 5372

Citations 39

Authors

P A Mardh

L Westtom

Affiliations

Soon will be listed here.

Abstract

Vaginal epithelial cells from healthy women were washed and incubated in tissue culture medium with freshly isolated bacteria of the indigenous vaginal flora and with bacteria of species that have been discussed in conjunction with genital infections. After incubation and washing, the number of bacteria that adhered per cell was determined. The influence on the attachment rate of such factors as variations in the washing procedure, bacterial density, and incubation time was assessed. Lactobacillus acidophilus and other bacterial species that occur in the lower genital tract of healthy women, e.g., some strictly anaerobic species, adhered by significantly lower numbers per cell than Neisseria gonorrhoeae, group B streptococci, and Corynebacterium vaginale. Significantly more freshly isolated gonococci adhered per cell than gonococci that had been passaged on artificial medium. The adherence of gonococci increased with increasing acidity of the test medium.

Citing Articles

Development of methods to model the impact of vaginal lactobacilli on biofilm formation on menstrual cups as well as validation of recommended cleaning directions.

Friberg M, Woeller K, Iberi V, Mancheno P, Riedeman J, Bohman L Front Reprod Health. 2023; 5:1162746.

PMID: 37671283 PMC: 10475951. DOI: 10.3389/frph.2023.1162746.

Examination of type IV pilus expression and pilus-associated phenotypes in Kingella kingae clinical isolates.

Kehl-Fie T, Porsch E, Yagupsky P, Grass E, Obert C, Benjamin Jr D Infect Immun. 2010; 78(4):1692-9.

PMID: 20145101 PMC: 2849430. DOI: 10.1128/IAI.00908-09.

Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae.

Wu H, Seib K, Srikhanta Y, Edwards J, Kidd S, Maguire T J Proteomics. 2009; 73(5):899-916.

PMID: 20004262 PMC: 4238106. DOI: 10.1016/j.jprot.2009.12.001.

Assessment of vaginal lactobacillary flora in wet mount and fresh or delayed gram's stain.

Donders G, Vereecken A, Salembier G, Van Bulck B, Spitz B Infect Dis Obstet Gynecol. 1996; 4(1):2-6.

PMID: 18476056 PMC: 2364465. DOI: 10.1155/S1064744996000026.

Influence of ovarian hormones on urogenital infection.

Sonnex C Sex Transm Infect. 1998; 74(1):11-9.

PMID: 9634294 PMC: 1758080. DOI: 10.1136/sti.74.1.11.

References

Mardh P, Westrom L, Akerlund M . In vitro experiments on adherence of bacteria to vaginal epithelial cells. Acta Obstet Gynecol Scand. 1975; 54(2):193-4. DOI: 10.3109/00016347509156756. View

Parsons C, Greenspan C, Mulholland S . The primary antibacterial defense mechanism of the bladder. Invest Urol. 1975; 13(1):72-8. View

Gibbons R, Houte J . Bacterial adherence in oral microbial ecology. Annu Rev Microbiol. 1975; 29:19-44. DOI: 10.1146/annurev.mi.29.100175.000315. View

Swanson J, Buchanan T, WENDE R, Williams R . Differential attachment by piliated and nonpiliated Neisseria gonorrhoeae to human sperm. Infect Immun. 1974; 9(5):897-902. PMC: 414903. DOI: 10.1128/iai.9.5.897-902.1974. View

Christensen K, Christensen P, Flamholc L, Ripa T . Frequencies of streptococci of groups A, B, C, D, and G in urethra and cervix swab specimens from patients with suspected gonococcal infection. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974; 82(4):470-4. DOI: 10.1111/j.1699-0463.1974.tb02355.x. View

Gibbons R, van Houte J, Liljemark W . Parameters that effect the adherence of Streptococcus salivarius to oral epithelial surfaces. J Dent Res. 1972; 51(2):424-35. DOI: 10.1177/00220345720510023101. View

van Houte J, Gibbons R, Pulkkinen A . Ecology of human oral lactobacilli. Infect Immun. 1972; 6(5):723-9. PMC: 422600. DOI: 10.1128/iai.6.5.723-729.1972. View

Liljemark W, Gibbons R . Proportional distribution and relative adherence of Streptococcus miteor (mitis) on various surfaces in the human oral cavity. Infect Immun. 1972; 6(5):852-9. PMC: 422620. DOI: 10.1128/iai.6.5.852-859.1972. View

WILKINSON H, Facklam R, Wortham E . Distribution by serological type of group B streptococci isolated from a variety of clinical material over a five-year period (with special reference to neonatal sepsis and meningitis). Infect Immun. 1973; 8(2):228-35. PMC: 422837. DOI: 10.1128/iai.8.2.228-235.1973. View

10.

DUNKELBERG Jr W, Skaggs R, KELLOGG Jr D . Method for isolation and identification of Corynebacterium vaginale (Haemophilus vaginalis). Appl Microbiol. 1970; 19(1):47-52. PMC: 376607. DOI: 10.1128/am.19.1.47-52.1970. View

11.

Liljemark W, Gibbons R . Ability of Veillonella and Neisseria species to attach to oral surfaces and their proportions present indigenously. Infect Immun. 1971; 4(3):264-8. PMC: 416298. DOI: 10.1128/iai.4.3.264-268.1971. View

12.

Cohen L . Influence of pH on vaginal discharges. Br J Vener Dis. 1969; 45(3):241-7. PMC: 1048473. DOI: 10.1136/sti.45.3.241. View

13.

EYER H . [On the biology and bacteriology of the vaginal secretion]. Arch Gynakol. 1961; 195:11-20. DOI: 10.1007/BF00815546. View

14.

Abrams G, Bauer H, SPRINZ H . Influence of the normal flora on mucosal morphology and cellular renewal in the ileum. A comparison of germ-free and conventional mice. Lab Invest. 1963; 12:355-64. View

15.

REYN A . LABORATORY DIAGNOSIS OF GONOCOCCAL INFECTIONS. Bull World Health Organ. 1965; 32:449-69. PMC: 2555271. View

16.

Gardner H, DUKES C . Haemophilus vaginalis vaginitis: a newly defined specific infection previously classified non-specific vaginitis. Am J Obstet Gynecol. 1955; 69(5):962-76. View

17.

Gibbons R, van Houte J . Selective bacterial adherence to oral epithelial surfaces and its role as an ecological determinant. Infect Immun. 1971; 3(4):567-73. PMC: 416198. DOI: 10.1128/iai.3.4.567-573.1971. View