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Method for Fingerprinting Yeast Cell Wall Mannans

Overview
Journal J Bacteriol
Specialty Microbiology
Date 1969 Dec 1
PMID 5361210
Citations 88
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Abstract

Controlled acetolysis of yeast mannans yields mixtures of oligosaccharides with (1-->2) and (1-->3) linkages between the mannose units, whereas the less stable (1-->6) linkages of the polysaccharide backbone are cleaved. The "fingerprints," obtained by gel filtration of the oligosaccharide mixtures, can be used to distinguish between the different yeast mannans. The general method may be useful for determining the taxonomy of yeasts and for making correlations between immunochemical reactivity and mannan structure.

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